Study on simultaneous qualitative and quantitative dosage of morphine and codeine by HPLC
- Author:
Lau Van Trinh
- Publication Type:Journal Article
- Keywords:
morphine
- MeSH:
Morphine;
Codeine
- From:Pharmaceutical Journal
2000;290(6):25-26
- CountryViet Nam
- Language:Vietnamese
-
Abstract:
After extracting completely morphine and codeine from liquid extract of opinum with a mixture of 3 volumes of isopropanol and 7 volumes of chloroform, an HPLC method was used to determine simultaneously morphine and codeine in the extracts. The HPLC technique was carried out on the column lichrosorb (4x250mm, 10 Mm) with diode array detector or UV- detector at landa = 285 nm and a mixture of 42 volumes of 0.24% sodium heptanesulfonat solution adjusted to pH = 3.2 by phosphoric acid and 18 volumes of Acetonitrile as mobile phase at flow rate 1.0 ml/min. The experimental results proved that the proposed HPLC method was rapid, specific, accurate and precise.
