Induction of IL-8 and reactive oxygen species in periodontal ligament cells by Aggregatibacter actinomycetemcomitans.
10.5051/jkape.2009.39.3.331
- Author:
Yang Sin LEE
1
;
Hong Gyu PARK
;
Sung Whan KIM
;
Jeong Heon CHA
;
Yun Jung YOO
Author Information
1. Department of Oral Biology, College of Dentistry, Yonsei University, Seoul, Korea. yu618@yuhs.ac
- Publication Type:Original Article
- Keywords:
Aggregatibacter actinomycetemcomitans;
interleukin-8;
periodontal ligament;
reactive oxygen species
- MeSH:
Cytokines;
Enzyme-Linked Immunosorbent Assay;
Fluoresceins;
Interleukin-8;
Interleukins;
Periodontal Ligament;
Reactive Oxygen Species
- From:The Journal of the Korean Academy of Periodontology
2009;39(3):331-337
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: Interleukin (IL)-8 is one of pro-inflammatory cytokines. Reactive oxygen species (ROS) are reduced metabolites of O2. Aggregatibacter actinomycetemcomitans is one of representative periodontopathogens. To investigate the role of A. actinomycetemcomitans in IL-8 expression of periodontal ligament (PDL) cells, we estimated the production of IL-8 and ROS in A. actinomycetemcomitans treated PDL cells. METHODS: The IL-8 production was determined by enzyme-linked immunosorbent assay. The ROS production was estimated using H2DCFDA and FACS. RESULTS: A. actinomycetemcomitans increased the production of IL-8 and ROS at 10, 100, and 500 multiplicity of infection. N-cetylcysteine, an antioxidant of ROS, down-regulated the production of IL-8 induced by A. actinomycetemcomitans. CONCLUSION: These results suggest that A. actinomycetemcomitans induces IL-8 production and ROS may act as a mediator in this process.
