Response of fetal rat calvarial cells on mineral trioxide aggregate after IL-1beta stimulation.
10.5051/jkape.2009.39.3.359
- Author:
Sool Heon LEE
1
;
Ji Il PARK
;
Young Joon KIM
Author Information
1. Department of Periodontology, School of Dentistry, Dental Science Reserch Institute, Chonnam National University, Gwang-Ju, Korea. youngjun@chonnam.ac.kr
- Publication Type:Original Article
- Keywords:
biocompatible materials;
cytokines;
ELISA;
mineral trioxide aggregate
- MeSH:
Aluminum Compounds;
Animals;
Biocompatible Materials;
Calcium Compounds;
Cell Proliferation;
Cytokines;
Drug Combinations;
Enzyme-Linked Immunosorbent Assay;
Glass;
Glutamates;
Guanine;
Interleukin-6;
Osteoclasts;
Oxides;
Rats;
RNA, Messenger;
Silicates;
Pemetrexed
- From:The Journal of the Korean Academy of Periodontology
2009;39(3):359-365
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: The purpose of this study was to investigate the ability of Mineral trioxide aggregate(MTA) to support osteoclastic differentiation from fetal rat calvarial cell. METHODS: In this study, response of IL-6, RANKL, and OPG in fetal rat calvarial cells stimulated with IL-1beta on MTA was evaluated by ELISA and RT-PCR. RESULTS: The results were as follows; there was no significant difference between glass and MTA at 5days. In ELISA analysis, Glass group and MTA group showed similar IL-6 expression, Glass+IL-1beta group and MTA+IL-1beta group showed similar IL-6 expression. In RT-PCR analysis, Glass group and MTA group showed similar IL-6, RANKL, OPG mRNA expression, MTA+IL-1beta group and Glass+IL-1beta group showed 3 fold increase of IL-6 and RNAKL mRNA expression when compared with MTA group. All groups showed similar OPG mRNA expression. CONCLUSIONS: MTA does not suppress cell proliferation and increase the proinflammatory cytokine that induce osteoclastogenesis. Thus, MTA is biocompatible material that could be used in various clinical conditions.
