Association of Polymorphisms in the IL-10 and IFN-gamma Genes with Allograft Dysfunction Following Kidney Transplantation in Koreans.
- Author:
Eun Youn ROH
1
;
Myoung Hee PARK
;
Hye jin PARK
;
Jongwon HA
;
Sang Joon KIM
;
Curie AHN
Author Information
1. Department of Laboratory Medicine, Seoul National University College of Medicine, Seoul, Korea. parkmhee@snu.ac.kr
- Publication Type:Original Article
- Keywords:
IL-10;
IFN-gamma;
Polymorphism;
Renal transplantation;
Acute rejection;
Chronic renal allograft dysfunction
- MeSH:
Alleles;
Allografts*;
Cytokines;
Gene Frequency;
Humans;
Incidence;
Interleukin-10*;
Introns;
Kidney Transplantation*;
Kidney*;
Organ Transplantation;
Promoter Regions, Genetic;
Transplants
- From:The Journal of the Korean Society for Transplantation
2003;17(1):34-42
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: IL-10 and IFN-gamma are amongst important cytokines, which are thought to have influence on organ transplantation outcome. The aim of this study was to investigate the IL-10 and IFN-gamma gene polymorphisms in Koreans, and their association with renal transplantation outcome. METHODS: Three SNP sites (-1082 G/A, -819 C/T, -592 C/A) of IL-10 promoter region and CA repeats in intron 1 of IFN-gamma gene were analyzed using PCR-single strand conformation polymorphism (SSCP) and direct sequencing methods in 73 controls and 164 kidney allograft recipients. Association between polymorphisms of these genes and transplantation outcome was analyzed using chi square test or Fisher's exact test. RESULTS: The allele frequencies of the IL-10 and IFN-gamma genes showed no significant differences between the control and patient groups. The frequencies of IL-10 and IFN-gamma high producer alleles were markedly lower than those of Caucasians. The incidence of multiple acute rejection episodes was higher in IL-10low producer (-1082 AA) than intermediate producer (-1082 GA) group (8.6% vs 0%), and in IFN-gamma high producer ([CA]12 positive) than low producer ([CA]12 negative) group (11.9% vs 6.6%). The incidence of chronic renal allograft dysfunction was lower in IL-10 intermediate producer than low producer group (7.7% vs 18.0%), and also lower in the combination of IL-10 intermediate/IFN-gamma low producer type than in other combinations (0% vs 18.2%). However, all these differences were not statistically significant. CONCLUSION: IL-10 and IFN-gamma have little influence on renal transplantation outcome in Koreans, probably due to quite limited polymorphisms of these genes in this population. The results of this study would be useful as basic data for renal transplantation in Koreans.
