PCR Approach for Detection and Typing of Epidermodysplasia Verruciformis-associated Human Papillomavirus Types.
- Author:
Yong Jae SHIN
;
Hyun Chul CHOI
;
Chan Gil KIM
;
Kwang Ho LEE
- Publication Type:Original Article
- Keywords:
Retroviral vector;
Viral titer;
CD4;
CD8
- MeSH:
Clone Cells;
Dermatoglyphics;
Digestion;
DNA;
Humans*;
Mucous Membrane;
Polymerase Chain Reaction*;
Skin;
Warts
- From:Journal of the Korean Society of Virology
1999;29(1):39-44
- CountryRepublic of Korea
- Language:English
-
Abstract:
Warts, or verrucae, are benign epithelial proliferations of the skin and mucosa caused by infection with human papillomaviruses (HPV). It is now recognized that there are many different HPV types. Especially type3 is most frequently observed in flat wart. Other types, such as type2, 10, 14, 27, 28, 29, 38, and 41 are rarely encounted in flat wart. We describe here a simple and economic method for detection and identification of epidermodysplasia verruciformis-associated HPV. The method is based on polymerase chain reaction (PCR) amplification and restriction analysis. The method has been developed with cloned HPV DNA and DNA from clinical samples. Clinical samples are from either frozen tissue or paraffin-embedded tissue. Genomic fragments were obtained from two different HPV types (3 and 10). The amplification fragments were identified by a form of miniature fingerprinting, with a set of restriction enzymes that gave a unique digestion pattern for each HPV type. We have tested 74 clinical samples. Only type3 among these clinical samples is detected, and one sample is involved in neither type3 nor type10.
