The Phylogenetic Analysis of the NS-5 Region Sequence of Hepatitis G Viruses Isolated in Korea.
- Author:
Youngmee JEE
;
Ki Soon KIM
;
Doo Sung CHEON
;
Jeong Koo PARK
;
Young Hwa KANG
;
Yoon Sung LEE
;
Yoon Suk CHUNG
;
Ji Eun KIM
- Publication Type:Original Article
- Keywords:
Hepatitis G virus;
NS-5 region;
Phylogenetic analysis
- MeSH:
Clone Cells;
Cloning, Organism;
DNA, Complementary;
GB virus C*;
Hand;
Hepatitis*;
Humans;
Korea*;
Polymerase Chain Reaction;
RNA, Viral
- From:Journal of the Korean Society of Virology
1999;29(1):45-53
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
We examined the hepatitis G virus infections among 227 Koreans who were healthy or were suspected of hepatitis and determined the phylogenetic relationship based on a part of the NS-5 region of 5 positive samples. Viral RNA was extracted from sera and cDNA was synthesized and subsequently amplified by RT-PCR (reverse transcription-polymerase chain reaction) or RT-nested PCR using random hexamer and NS-5 specific primers (470-20-1-77F, 470-20-1-211R, HGVNESTFO, HGVNESTRE). Five positives were found to belong to samples of patients showing symptoms of viral hepatitis. Primers used for PCR or nested PCR were derived from the NS-5 region. On the other hand, no amplification was detected using primers derived from the 5'-NCR (G-146F, G-401R). We performed TA cloning and sequencing of 5 amplified fragments, and their sequences were compared with those of foreign isolates of HGV. The phylogenetic analysis using MegAlign programme of DNAstar has shown that the Korean isolates are clustered on the phylogenetic tree. In summary, we confirmed the hepatitis G virus infection in 5 cases out of 12 patients showing the symptoms of viral hepatitis. The phylogenetic analysis of sequences of 5 amplified fragments showed that their relations to each other were closer than those to the foreign HGV isolates reported.
