The Relationship between Gastric Juice Nitrate/Nitrite Concentrations and Gastric Mucosal Surface pH.
10.3349/ymj.2012.53.6.1154
- Author:
Hae Kyung LEE
1
;
Hyunjung KIM
;
Hyung Keun KIM
;
Young Seok CHO
;
Byung Wook KIM
;
Sok Won HAN
;
Lee So MAENG
;
Hiun Suk CHAE
;
Hee Na KIM
Author Information
1. Department of Laboratory Medicine, The Catholic University of Korea College of Medicine, Seoul, Korea.
- Publication Type:Original Article
- Keywords:
Nitrate/nitrite;
gastric mucosa surface pH;
gastric juice pH;
chromoscopy;
Helicobacter pylori
- MeSH:
Adult;
Aged;
Dyspepsia/*metabolism/microbiology;
Enzyme-Linked Immunosorbent Assay;
Female;
Gastric Juice/*metabolism;
Gastric Mucosa/*metabolism/microbiology;
Helicobacter pylori/isolation & purification;
Humans;
Hydrogen-Ion Concentration;
Male;
Middle Aged;
Nitrates/*metabolism;
Nitrites/*metabolism;
Young Adult
- From:Yonsei Medical Journal
2012;53(6):1154-1158
- CountryRepublic of Korea
- Language:English
-
Abstract:
PURPOSE: To investigate gastric juice nitrate/nitrite concentration according to mucosal surface pH extent (area) of gastric corpus intimately contacting the gastric juice. MATERIALS AND METHODS: We included ninety-nine patients with dyspepsia. To evaluate gastric mucosal surface pH and its extent, gastric chromosocpy was performed by spraying phenol red dye on the corpus mucosa and estimating the extent of area with color changed. Nitrate/nitrite concentrations and pH of gastric juice were measured by ELISA and pH meter, respectively. Silver staining was done to histologically confirm the presence of Helicobacter pylori. RESULTS: Intragastric nitrate/nitrite concentrations in patients, showing phenol red staining mucosa were higher than those of unstaining mucosa (p=0.001): the more extensive in the area of phenol red staining area of corpus, the higher gastric juice pH found (r=0.692, p<0.001). Furthermore, the intragastric nitrate/nitrite concentrations correlated positively with gastric juice pH (r=0.481, p<0.001). CONCLUSION: The changes of mucosal surface pH and its extent in gastric corpus might affect either pH or nitrate/nitrite level of gastric juice.
