Expression of Cyclin D1, Cyclin E, p21Cip1 and p27Kip1 in Chemically Induced Rat Mammary Tumor Treated with Tamoxifen and Transforming Growth Factor-1.
- Author:
Tae Jung JANG
1
;
Jae Hum PARK
;
Mee Yon CHO
;
Ki Kwon KIM
;
Jung Ran KIM
Author Information
1. Department of Pathology, Dongguk University College of Medicine, Kyungju 780-714, Korea. taejung@mail.dongguk.ac.kr
- Publication Type:Original Article
- Keywords:
Breast;
Neoplasms;
Cell cycle;
Tamoxifen;
Transforming growth factor beta
- MeSH:
Animals;
Blotting, Western;
Breast;
Cell Cycle;
Cyclin D1*;
Cyclin E*;
Cyclins*;
Endothelial Cells;
Estrogens;
Hand;
Immunohistochemistry;
Rats*;
Receptors, Estrogen;
Robenidine;
Tamoxifen*;
Transforming Growth Factor beta
- From:Korean Journal of Pathology
2001;35(2):151-157
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: Tamoxifen (TAM) inhibits the action of estrogen by binding to estrogen receptors, and also has non-estrogen receptor mediated cytostatic activities. Transforming growth factor-1 (TGF-1) inhibits the proliferation of many other cell types, such as epithelial, hematopoietic and endothelial cells. METHODS: We investigated the effects of tamoxifen on the growth of 7,12-dimethylbenz(a)anthracene (DMBA)-induced rat mammary tumors and the expression of cyclin D1, cyclin E, p21Cip1, and p27Kip1 by performing immunohistochemistry and Western blot analysis, and studied whether TGF-1 injection amplified the effects of TAM. When tumor size reached between 10-15 mm in the largest dimension, the rats were divided into 3 groups: DMBA-control group (n=12), DMBA-TAM group (n=14) and DMBA-TAM plus TGF-1 group (n=5). RESULTS: The consecutive administration of TAM markedly decreased the tumor development compared with the DMBA-control group. The DMBA-TAM and DMBA-TAM plus TGF-1 groups showed decreased expression of bromodexoyuridine, cyclin D1, cyclin E, and p21Cip1 when compared with those of the DMBA-control group. On the other hand, the labeling index of p27Kip1 was higher in the DMBA-TAM plus TGF-1 group than in the DMBA-control group. CONCLUSION: TAM suppresses tumor development, which may be associated with down-expression of cyclin D1 and cyclin E, and overexpression of p27Kip1, and addition of TGF-1 does not influence tumor development treated by TAM.
