E-cadherin and beta-catenin Expression and Mutation in Gastric Carcinomas.
10.5230/jkgca.2001.1.4.202
- Author:
Kwang Il KIM
1
;
Sung Hye PARK
;
Sun Ae HAN
;
Yang Seok CHAE
;
Insun KIM
Author Information
1. Department of Pathology, Korea University Medical College, Seoul, Korea. iskim@korea.ac.kr
- Publication Type:Original Article
- Keywords:
Gastric carcinoma;
E-cadherin;
beta-catenin;
Immunohistochemistry
- MeSH:
Adenocarcinoma;
beta Catenin*;
Cadherins*;
Carcinogenesis;
Carcinoma, Signet Ring Cell;
Cytoplasm;
Immunohistochemistry;
Lymph Nodes;
Neoplasm Metastasis;
Stomach
- From:Journal of the Korean Gastric Cancer Association
2001;1(4):202-209
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: When cancer cels invade the stroma, they should be dissociated from the adjacent cells at first. E-cadherin and beta-catenin constitute an important protein complex associated with cellular adhesion, development, and differentiation, especially in epithelial cells.The role of E-cadherin and beta- catenin in gastric carcinogenesis were studied. MATENRIALS AND METHODS: The expression of E-cadherin and beta-catenin in gastric adenocarcinomas by using immunohistochemical staining and the mutation by using polymerase chain reaction- single stranded conformation polymorphism (PCR-SSCP) and sequencing were performed in 40 adenocarcinomas and 5 dysplasia of stomach. Thirteen cases, which had lymph node metastasis, were also included for immunohistochemical staining. RESULTS: Inappropriate cytoplasmic and/or nuclear expression of a E-cadherin-beta-catenin complex was more frequent in poorly differentiated, diffuse type signet ring cell carcinomas than in well-differentiated, intestinal type adenocarcinomas (P<0.05). However, the expression was not related with clinical stage or lymph node metastasis. Mutation of E-cadherin was detected in 4 cases by using PCR-SSCP, whereas mutation of beta-catenin was detected in 2 cases. CONCLUSION: E-cadherin and beta-catenin seem to be important in gastric carcinogenesis, especially in poorly differentiated diffuse type.
