Collagen Synthesis by RPE Cells Associated with Proliferative Vitreoretinopathy.
- Author:
Sung Chul LEE
1
;
Oh Woong KWON
;
Soon Hyun KIM
Author Information
1. Institute of Vision Research, Yonsei University College of Medicine, Seoul, Korea.
- Publication Type:Original Article
- Keywords:
Collagen;
Proliferative vitreoretinopathy;
Retinal pigment epithelium;
TGRbeta
- MeSH:
Collagen*;
Electrophoresis;
Extracellular Matrix;
Membranes;
Retinal Pigment Epithelium;
Transforming Growth Factor beta;
Transforming Growth Factors;
Vitreoretinopathy, Proliferative*
- From:Journal of the Korean Ophthalmological Society
1998;39(8):1801-1807
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
Collagen, which is newly synthesized by the cellular component, is the major component of extracellular matrix in PVR membranes. The effects of transforming growth factor (TGF)-beta in the synthesis of collagen were evaluated. Tissue culture of PVR membrane was incubated for 19 hours with or without TGF-beta in the absence of serum. Newly synthesized collagen in the membrane were measured by a collagenase-digestion assay. The biosynthetically labeled collagens were analyzed on SDS-polyacrylamide gel electrophoresis. Collagen synthesis was dose-dependently stimulated by TGFbeta. At a concentration of 1ng/ml of TGFbeta1or TGFbeta2,at a concentration of 10ng/ml, induced a 90125% increase in collagen synthesis over that of controls. PVR membranes in culture synthesized predominantly type I, III and V collagen. In conclusion TGFbeta are potent stimulants of collagen.
