Identification of Dammarane-type Triterpenoid Saponins from the Root of Panax ginseng.
- Author:
Dong Gu LEE
1
;
Jaemin LEE
;
Sanghoon YANG
;
Kyung Tack KIM
;
Sanghyun LEE
Author Information
1. Department of Integrative Plant Science, Chung-Ang University, Anseong 456-756, Korea. slee@cau.ac.kr
- Publication Type:Original Article
- Keywords:
Panax ginseng;
Ginsenoside;
Dammarane-type triterpenoid;
Saponin
- MeSH:
Chromatography;
Chromatography, Liquid;
Ginsenosides;
Magnetic Resonance Spectroscopy;
Medicine, Korean Traditional;
Panax*;
Saponins*
- From:Natural Product Sciences
2015;21(2):111-121
- CountryRepublic of Korea
- Language:English
-
Abstract:
The root of Panax ginseng, is a Korea traditional medicine, which is used in both raw and processed forms due to their different pharmacological activities. As part of a continued chemical investigation of ginseng, the focus of this research is on the isolation and identification of compounds from Panax ginseng root by open column chromatography, medium pressure liquid chromatography, semi-preparative-high performance liquid chromatography, Fast atom bombardment mass spectrometric, and nuclear magnetic resonance. Dammarane-type triterpenoid saponins were isolated from Panax ginseng root by open column chromatography, medium pressure liquid chromatography, and semi-preparative-high performance liquid chromatography. Their structures were identified as protopanaxadiol ginsenosides [gypenoside-V (1), ginsenosides-Rb1 (2), -Rb2 (3), -Rb3 (4), -Rc (5), and -Rd (6)], protopanaxatriol ginsenosides [20(S)-notoginsenoside-R2 (7), notoginsenoside-Rt (8), 20(S)-O-glucoginsenoside-Rf (9), 6-O-[alpha-L-rhamnopyranosyl(1-->2)-beta-D-glucopyranosyl]-20-O-beta-D-glucopyranosyl-3beta,12beta, 20(S)-dihydroxy-dammar-25-en-24-one (10), majoroside-F6 (11), pseudoginsenoside-Rt3 (12), ginsenosides-Re (13), -Re5 (14), -Rf (15), -Rg1 (16), -Rg2 (17), and -Rh1 (18), and vinaginsenoside-R15 (19)], and oleanene ginsenosides [calenduloside-B (20) and ginsenoside-Ro (21)] through the interpretation of spectroscopic analysis. The configuration of the sugar linkages in each saponin was established on the basic of chemical and spectroscopic data. Among them, compounds 1, 8, 10, 11, 12, 19, and 20 were isolated for the first time from P. ginseng root.
