IL-10 is Predominantly Produced by CD19(low)CD5(+) Regulatory B Cell Subpopulation: Characterisation of CD19 (high) and CD19(low) Subpopulations of CD5(+) B cells.
10.3349/ymj.2011.52.5.851
- Author:
Jae Ho LEE
1
;
Joonyong NOH
;
Geunwoong NOH
;
Wahn Soo CHOI
;
Sang Sun LEE
Author Information
1. Department of Paediatrics, College of Medicine, Chungnam National University, Daejeon, Korea.
- Publication Type:Brief Communication ; Research Support, Non-U.S. Gov't
- Keywords:
Regulatory B cell;
CD5;
CD19;
IL-10;
CD5(+) B
- MeSH:
Antigens, CD19/metabolism;
Antigens, CD5/metabolism;
Apoptosis/immunology;
B-Lymphocyte Subsets/cytology/*immunology;
Cell Separation;
Flow Cytometry;
Humans;
Interleukin-10/*biosynthesis
- From:Yonsei Medical Journal
2011;52(5):851-855
- CountryRepublic of Korea
- Language:English
-
Abstract:
IL-10 production by CD19(+)CD5(+) B cells was investigated, by determining the expression levels of CD19, a classical B cell marker. Peripheral mononuclear cells were stained with fluorescence-conjugated anti-CD5, anti-CD19, anti-IL-10, and Annexin V. Interestingly, IL-10-producing B cells were found to be localised within the CD19(low)CD5(+) B cell subset. Apoptotic changes were also observed mainly in CD19(low) cells among B cells. Thus, CD5(+) B cells should be classified as CD19(high) and CD19(low) cells, and the immunological significance of CD19 for the IL-10 production by CD5(+) B cells requires further studies.
