Role of interleukin-6 in orthodontically induced inflammatory root resorption in humans.
10.4041/kjod.2013.43.6.294
- Author:
Ryuichi KUNII
1
;
Masaru YAMAGUCHI
;
Yasuhiro TANIMOTO
;
Masaki ASANO
;
Kunihiko YAMADA
;
Takemi GOSEKI
;
Kazutaka KASAI
Author Information
1. Department of Orthodontics, Nihon University School of Dentistry at Matsudo, Chiba, Japan. yamaguchi.masaru@nihon-u.ac.jp
- Publication Type:In Vitro ; Original Article
- Keywords:
Interleukin-6;
Osteo/odontoclastogenesis;
Orthodontic force;
Root resorption
- MeSH:
Culture Media, Conditioned;
Dentin;
Enzyme-Linked Immunosorbent Assay;
Gingival Crevicular Fluid;
Humans*;
Interleukin-6*;
Interleukins;
Osteoclasts;
Periodontal Ligament;
Real-Time Polymerase Chain Reaction;
RNA, Messenger;
Root Resorption*
- From:The Korean Journal of Orthodontics
2013;43(6):294-301
- CountryRepublic of Korea
- Language:English
-
Abstract:
OBJECTIVE: To determine the interleukin (IL)-6 levels in gingival crevicular fluid (GCF) of patients with severe root resorption after orthodontic treatment and investigate the effects of different static compressive forces (CFs) on IL-6 production by human periodontal ligament (hPDL) cells and the influence of IL-6 on osteoclastic activation from human osteoclastic precursor (hOCP) cells in vitro. METHODS: IL-6 levels in GCF samples collected from 20 patients (15 and 5 subjects without and with radiographic evidence of severe root resorption, respectively) who had undergone orthodontic treatment were measured by ELISA. The levels of IL-6 mRNA in hPDL cells and IL-6 protein in conditioned medium after the application of different uniform CFs (0, 1.0, 2.0, or 4.0 g/cm2 for up to 72 h) were measured by real-time PCR and ELISA, respectively. Finally, the influence of IL-6 on mature osteoclasts was investigated by using hOCP cells on dentin slices in a pit-formation assay. RESULTS: Clinically, the IL-6 levels were significantly higher in the resorption group than in the control group. In vitro, IL-6 mRNA expression significantly increased with increasing CF. IL-6 protein secretion also increased in a time- and magnitude-dependent manner. Resorbed areas on dentin slices were significantly greater in the recombinant human IL-6-treated group and group cultured in hPDL cell-conditioned medium with CF application (4.0 g/cm2) than in the group cultured in hPDL cell-conditioned medium without CF application. CONCLUSIONS: IL-6 may play an important role in inducing or facilitating orthodontically induced inflammatory root resorption.
