Quantification of endothelin-1 in human umbilical venous endothelial cell culture supernatants of small for gestational age and preeclampsia neonates.
10.3345/kjp.2007.50.12.1194
- Author:
Won Kyoung CHO
1
;
So Young KIM
;
Chung Sik CHUN
Author Information
1. Department of Pediatrics, College of Medicine, Catholic University, Seoul, Korea. sykimped@catholic.ac.kr
- Publication Type:Original Article
- Keywords:
Preeclampsia;
Small for gestational age;
Endothelin-1;
Umbilical vein;
Endothelial cell
- MeSH:
Adult;
Endothelial Cells*;
Endothelin-1*;
Fetal Blood;
Fetal Growth Retardation;
Gestational Age*;
Humans*;
Infant, Newborn*;
Mothers;
Pre-Eclampsia*;
Umbilical Veins
- From:Korean Journal of Pediatrics
2007;50(12):1194-1199
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: It was generally accepted now a days that the pathogenesis of preeclampsia, small for gestational age (SGA), intrauterine growth retardation and fetal origin of adult diseases were related with a endothelial cell dysfunction. The purpose of this study was to know the relation of such diseases by assessing the level of endothelin-1. METHODS: SGA babies, newborns of preeclampsia and normal control mother were included in this study. Isolated endothelial cells were centrifugated and mixed with media in 37, 5% CO2 to obtain confluent monolayer of cultured human umbilical venous endothelial cell (HUVEC). Endothelin-1 levels were determined by Endothelin-1 colorimetric (EIA) Kits. We examined the endothelin-1 level in the HUVEC supernatants from SGA baby, and newborns from preeclampsia as well as normal mother. Also, we compared the endothelin-1 level of cultured normal HUVEC incubated with serum from cord blood of SGA, babies of preeclampsia or normal control mother. RESULTS: The endothelin-1 levels in cultured HUVEC supernatants of three groups showed no significant difference but the endothelin-1 levels of cultured normal HUVEC incubated with serum from preeclampsia mother or SGA mother was significantly higher than those from newborns of control mothers (P<0.05). CONCLUSION: These findings suggest that there may be the factor which affect the endothelin-1 level in serum of cord blood from SGA and preeclampsia.
