Protease-Activated Receptor 2 Is Involved in Th2 Responses against Trichinella spiralis Infection.
10.3347/kjp.2011.49.3.235
- Author:
Mi Kyung PARK
1
;
Min Kyoung CHO
;
Shin Ae KANG
;
Hye Kyung PARK
;
Yun Seong KIM
;
Ki Uk KIM
;
Soon Cheol AHN
;
Dong Hee KIM
;
Hak Sun YU
Author Information
1. Department of Parasitology, School of Medicine, Pusan National University, Yangsan 626-870, Korea. hsyu@pusan.ac.kr
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
Trichinella spiralis;
protease-activated receptor 2;
IL-25;
thymic stromal lymphopoietin (TSLP)
- MeSH:
Animals;
Antibodies, Helminth/blood;
Chemokine CCL11/biosynthesis;
Cytokines/biosynthesis;
Female;
Gene Expression Profiling;
Immunoglobulin E/blood;
Interleukin-13/secretion;
Interleukin-4/secretion;
Interleukin-5/secretion;
Interleukins/biosynthesis;
Mice;
Mice, Inbred C57BL;
Mice, Knockout;
Receptor, PAR-2/*metabolism;
Th2 Cells/*immunology;
Trichinella spiralis/*immunology;
Trichinellosis/*immunology
- From:The Korean Journal of Parasitology
2011;49(3):235-243
- CountryRepublic of Korea
- Language:English
-
Abstract:
In order to get a better understanding of the role of protease-activated receptor 2 (PAR2) in type 2 helper T (Th2) cell responses against Trichinella spiralis infection, we analyzed Th2 responses in T. spiralis-infected PAR2 knockout (KO) mice. The levels of the Th2 cell-secreted cytokines, IL-4, IL-5, and IL-13 were markedly reduced in the PAR2 KO mice as compared to the wild type mice following infection with T. spiralis. The serum levels of parasite-specific IgE increased significantly in the wild type mice as the result of T. spiralis infection, but this level was not significantly increased in PAR2 KO mice. The expression level of thymic stromal lymphopoietin, IL-25, and eotaxin gene (the genes were recently known as Th2 response initiators) of mouse intestinal epithelial cells were increased as the result of treatment with T. spiralis excretory-secretory proteins. However, the expression of these chemokine genes was inhibited by protease inhibitor treatments. In conclusion, PAR2 might involve in Th2 responses against T. spiralis infection.
