In Vitro Effects of Some Herbs Used in Egyptian Traditional Medicine on Viability of Protoscolices of Hydatid Cysts.
10.3347/kjp.2011.49.3.255
- Author:
Doaa A YONES
1
;
Gamal A TAHER
;
Zedan Z IBRAHEIM
Author Information
1. Department of Medical Parasitology, Faculty of Medicine, Assiut University, Egypt. doaayones@gmail.com
- Publication Type:Original Article
- Keywords:
Echinococcus granulosus;
cystic echinococcus;
protoscolex;
thyme;
salvia;
essential oil;
thymol;
menthol
- MeSH:
Animals;
Anthelmintics/isolation & purification/*pharmacology;
Camels;
Cell Survival/drug effects;
Echinococcosis, Pulmonary/*parasitology;
Echinococcus granulosus/*drug effects/isolation & purification;
Lung/parasitology;
Medicine, Traditional/methods;
Plant Extracts/isolation & purification/*pharmacology;
Plants, Medicinal/*chemistry;
Salvia officinalis/chemistry;
Thymus Plant/chemistry;
Time Factors
- From:The Korean Journal of Parasitology
2011;49(3):255-263
- CountryRepublic of Korea
- Language:English
-
Abstract:
The present work evaluated the effects of alcoholic extracts of salvia (Salvia officinalis), thyme (Thymus vulgaris), and 2 pure compounds (thymol and menthol) on the viability of Echinococcus granulosus protoscolices in vitro. Four different concentrations of each extract (2,500, 1,500, 1,000, and 500 microg/ml) and 3 different concentrations each of thymol and menthol (50, 10, and 1 microg/ml) were used. Concentration of 2,500 microg/ml of both extracts showed a significant protoscolicidal activity on the 6th day. Complete loss of viability of protoscolices occurred with 500 microg/ml concentration of both extracts at day 6 and day 7 post-treatment (PT), respectively. Pure compounds, i.e., menthol and thymol, showed potent effects with 50 microg/ml concentration at day 2 and day 5 PT, respectively. These effects were compared with those of albendazole sulfoxide (800 microg/ml), a commonly used treatment drug for hydatidosis. Krebs-Ringer solution and the hydatid cystic fluid at a ratio of 4:1 was a good preservative solution which kept the protoscolices viable for 15 days.
