PCR Diagnosis of Entamoeba histolytica Cysts in Stool Samples.
10.3347/kjp.2011.49.3.281
- Author:
Joung Ho MOON
1
;
Shin Hyeong CHO
;
Jae Ran YU
;
Won Ja LEE
;
Hyeng Il CHEUN
Author Information
1. Division of Malaria and Parasitic Diseases, Korea National Institute of Health, Osong 363-951, Korea. ilcheun7@korea.kr
- Publication Type:Brief Communication ; Evaluation Studies ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
- Keywords:
Entamoeba histolytica;
SSU rDNA;
PCR;
diagnosis;
stool
- MeSH:
DNA Primers/genetics;
DNA, Protozoan/genetics;
DNA, Ribosomal/genetics;
Entamoeba histolytica/genetics/*isolation & purification;
Entamoebiasis/*diagnosis;
Histones/genetics;
Humans;
Molecular Diagnostic Techniques/*methods;
Parasitology/*methods;
Polymerase Chain Reaction/*methods;
Protozoan Proteins/genetics;
Sensitivity and Specificity
- From:The Korean Journal of Parasitology
2011;49(3):281-284
- CountryRepublic of Korea
- Language:English
-
Abstract:
Amebiasis is a protozoan disease caused by Entamoeba histolytica and a potential health threat in areas where sanitation and hygiene are inappropriate. Highly sensitive PCR methods for detection of E. histolytica in clinical and environmental samples are extremely useful to control amebiasis and to promote public health. The present study compared several primer sets for small subunit (SSU) rDNA and histone genes of E. histolytica cysts. A 246 bp of the SSU rDNA gene of pure cysts contained in phosphate-buffered saline (PBS) and in stool samples was successfully amplified by nested PCR, using the 1,147-246 bp primer set, of the primary PCR products which were pre-amplified using the 1,147 bp primer as the template. The detection limit of the nested PCR using the 1,147-246 primer set was 10 cysts in both groups (PBS and stool samples). The PCR to detect histone gene showed negative results. We propose that the nested PCR technique to detect SSU rDNA can be used as a highly sensitive genetic method to detect E. histolytica cysts in stool samples.
