NF-kappaB and CREB Are Involved in IL-8 Production of Human Neutrophils Induced by Trichomonas vaginalis-Derived Secretory Products.
10.3347/kjp.2011.49.3.291
- Author:
Young Hee NAM
1
;
Deulle MIN
;
Soon Jung PARK
;
Kyeong Ah KIM
;
Young Ah LEE
;
Myeong Heon SHIN
Author Information
1. Department of Environmental Medical Biology, Yonsei University College of Medicine, Seoul 120-752, Korea. myeong@yuhs.ac
- Publication Type:Brief Communication ; Research Support, Non-U.S. Gov't
- Keywords:
Trichomonas vaginalis;
human neutrophil;
IL-8;
NF-kappaB;
CREB
- MeSH:
Cyclic AMP Response Element-Binding Protein/*metabolism;
Human Experimentation;
Humans;
Interleukin-8/*metabolism;
Male;
NF-kappa B/*metabolism;
Neutrophils/*immunology;
Phosphorylation;
Trichomonas vaginalis/*immunology
- From:The Korean Journal of Parasitology
2011;49(3):291-294
- CountryRepublic of Korea
- Language:English
-
Abstract:
Trichomonas vaginalis is a flagellated lumen-dwelling extracellular protozoan parasite that causes human trichomoniasis via sexual intercourse. Human neutrophils play a crucial role in acute tissue inflammatory responses in T. vaginalis infection. In this study, we investigated the signaling mechanism of neutrophil responses when stimulated with T. vaginalis-derived secretory products (TvSP), which were collected from 1x10(7) live trichomonads. Incubation of human neutrophils isolated from peripheral blood with TvSP induced up-regulation of IL-8 protein secretion. In addition, stimulation with TvSP induced phosphorylation of NF-kappaB and CREB in neutrophils. Moreover, TvSP-induced IL-8 production was also significantly inhibited by pretreatment of neutrophils with ikappaB inhibitor or CREB inhibitor. These results suggest that transcription factors NF-kappaB and CREB are involved in IL-8 production in human neutrophils induced by stimulation with T. vaginalis infection.
