The Effect of Platelet Derived Growth Factor on the Myocardial Revascularization in Implantation of Rabbit Internal Mammary Artery.
- Author:
Yong Han KIM
1
;
Suk Joo RHA
;
Sang Hoon JUNG
;
Seok Whan MOON
;
Kuen Hyon JO
;
Young Pil WANG
;
Se Wha KIM
;
Moon Sub KWAK
Author Information
1. Department of Thoracic and Cardiovascular Surgery Catholic University College of Medicine, Seoul, Korea.
- Publication Type:Original Article
- Keywords:
Coronary artery bypass;
Myocondial infarction;
Internal thoracic artery;
Growth factor
- MeSH:
Blood Platelets*;
Coronary Artery Bypass;
Coronary Vessels;
Eosine Yellowish-(YS);
Hematoxylin;
Ligation;
Mammary Arteries*;
Mortality;
Myocardial Revascularization*;
Myocardium;
Necrosis;
Neutrophils;
New Zealand;
Platelet-Derived Growth Factor*;
Survival Rate;
Transplants
- From:The Korean Journal of Thoracic and Cardiovascular Surgery
2000;33(4):277-284
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: Coronary artery bypases grafting in the old aged is associated with high mortality and morbidity, and it is difficult to perform if the coronary artery is diffusely disease. Recently it has been known that platelet derived growth factor(PDGF), especially PDGF-BB, stimulates angiogenesis. MATERIAL AND METHOD: New Zealand white rabbit were used. In an attempt to achieve effevtive cardiac revasculatrization without vascular anastmosis, we divided into three groups(group I : Left anterior descending artery(LAD) was occluded by ligature, group II : Bilateral internal mammary vascular pedicles were dissected and implanted into myocardium, group III : The vascular pedicles were implanted into myocardium and PDGF-BB was injected into the myocardial tissue). Two weeks after IMA implantation, the proximal region of implanted LAD was ligated. Four days after LAD ligation angiogram, triphenyl tetrazolium chloride(TTD) staining and hematoxylin eosin staining were performed. RESULT: 1. Survival rate in group II was significantly higher than that in group I (P<0.05), and survival rate in group III was signficantly higher than that in group II(53% vs 93%, P<0.01). 2. There were significant differences in the ratio of area of necrosis to area at risk between group I and group II, and between group II and group III (P<0.01). 3. Microangiogram for angiogenic response revealed wide area of extensive revascularization with patent vessels in group III. 4. Histologic findings of three groups showed that polymorphonuclear leukocyte infiltration was minimal in group II and none in group III. CONCLUSIONS: PDGF-BB can establish functinal cardiac revasculatization through systemic vessels implanted directly into the myocardium.
