The Effect of Interleukin-1alpha on Trabecular Outflow Resistance in Rat Eyes.
- Author:
Changwon KEE
1
;
Kang Moon SEO
Author Information
1. Department of Ophthalmology, Samsung Medical Center, Seoul, Korea.
- Publication Type:Original Article
- Keywords:
Interleukin;
Outflow resistance;
Rat eye;
Matrix metallopro-teinase
- MeSH:
Animals;
Extracellular Matrix;
Interleukin-1alpha*;
Interleukins;
Pentobarbital;
Perfusion;
Rats*;
Serum Albumin, Bovine;
Trabecular Meshwork
- From:Journal of the Korean Ophthalmological Society
1997;38(9):1595-1599
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
It was investigated if regulation of the trabecular extracellular matrix tumover rate and remodeling plays an important role in decreasing outflow resistance by determining the effect of intracamerally given interleukin-1alpha a known stimulator of the expression of trabecular matrix metallopro-teinases, on outflow facility of albino rat eyes. Forty normal albino rats (Sprague dawley) weighing 250 to 300gm were studied. Rats were anesthetized by intraperitoneal pentobarbital sodium(30mg/kg) injection. The rats were grouped into 4 groups and given 5, 10, 25, 50 units of interleukin-1alpha injected intracamerally in one eye of each rat. Bovine serum albumin in phosphate buffered saline, which was used to dissolve the interleukin-1alpha, was injected in the other eye as a control. Outflow facility was measured by two level constant pressure perfusion at 1, 3, and 7 days after injection. The eyes treated with 50 units of interleukin-1alpha showed a statistically significant increase of outflow facility by 37% compared to the contralateral control eyes at 3 days after injection, but retumed to normal level in 7 days. The eyes treated with 5, 10, 25, 50 units of interleukin-1alpha increased the outflow facility, supporting the hypothesis that regulation of trabecular meshwork extracellular matrix plays a role in trabecular outflow resistance.
