Acanthopanax koreanum Nakai modulates the immune response by inhibiting TLR 4-dependent cytokine production in rat model of endotoxic shock.
- Author:
Myung Gi JUNG
1
;
Gyeong Min DO
;
Jae Ho SHIN
;
Young Min HAM
;
Soo Yeong PARK
;
Oran KWON
Author Information
- Publication Type:Original Article
- Keywords: Acanthopanax koreanum Nakai; acanthoic acid; toll-like receptor 4; inflammation; hepatotoxicity
- MeSH: Eleutherococcus*; Alanine Transaminase; Animals; Body Weight; Diterpenes; Hemorrhage; Hepatocytes; Inflammation; Interleukin-6; Liver; Liver Failure; Models, Animal*; Necrosis; Nitric Oxide; Plasma; Rats*; RNA, Messenger; Shock, Septic*; Toll-Like Receptor 4
- From:Nutrition Research and Practice 2013;7(6):460-465
- CountryRepublic of Korea
- Language:English
- Abstract: The hepatoprotective activity of Acanthopanax koreanum Nakai extract (AE) was investigated against D-Galactosamine/Lipopolysaccharide (D-GalN/LPS)-induced liver failure rats compared with that of acanthoic acid (AA) isolated from AE. Although D-GalN/LPS (250 mg/kg body weight/10 microg/kg body weight, i.p.) induced hepatic damage, pretreatments with AE (1 and 3% AE/g day) and AA (0.037% AA, equivalent to 3% AE/g day) alleviated the hepatic damage. This effect was the result of a significant decrease in the activity of alanine transaminase. Concomitantly, both the nitric oxide and IL-6 levels in the plasma were significantly decreased by high-dose AE (AE3) treatment compared to the GalN/LPS control (AE0). This response resulted from the regulation of pro-inflammatory signaling via a decrease in TLR4 and CD14 mRNA levels in the liver. While a high degree of necrosis and hemorrhage were observed in the AE0, pretreatment with AE3 and AA reduced the extent of hepatocyte degeneration, necrosis, hemorrhage and inflammatory cell infiltrates compared to the AE0. In conclusion, these results suggest that especially high-dose AE are capable of alleviating D-GalN/LPS-induced hepatic injury by decreasing hepatic toxicity, thereby mitigating the TLR 4-dependent cytokine release. The anti-inflammatory effect of AE could be contributing to that of AA and AE is better than AA.
