Species-Specific Detection of Listeria monocytogenes by Polymerase Chain Reaction.
- Author:
Byoung Seon YANG
1
;
Jin Sook PARK
Author Information
1. Department of Clinical Pathology, Chinju Junior College of Mursing and Health, Chinju, Korea.
- Publication Type:Original Article
- Keywords:
iap gene;
Listeria monocytogenes;
HRP;
nested PCR
- MeSH:
Blotting, Southern;
Cerebrospinal Fluid;
DNA;
Listeria monocytogenes*;
Listeria*;
Polymerase Chain Reaction*
- From:Journal of the Korean Society for Microbiology
1997;32(2):213-218
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
Three Listeria monocytogenes strains isolated from clinical cerebrospinal fluid samples. A PCR was performed by using oligonuclotide primers derived from the invasion-associated protein(iap) related gene sequence. As a result of PCR, we obtained 287-bp DNA fragment of specific product to L. monocytogenes species. The chromsomal DNA of genera Listeria species were subjected to southern blot hybridization with HRP-labeled 287-bp DNA probe to produce a L. monocytogenes banding pattern. In the cases of 10-fold dilution of L. monocytogenes cells, 8 x 10(2) cells/ml were detected by PCR.
