Study of dopamine transporter in the rat 6-hydroxydopamine Parkinson's disease model.
- Author:
Sang Yun KIM
1
;
Jae Min JUNG
;
Young Soo CHANG
;
Wan Seok JOO
;
Sung Ho MAENG
;
Chung Gun HA
;
Jong Min KIM
;
Yong Sik KIM
;
Myung Chul LEE
;
Sang Bok LEE
;
Beom S JEON
Author Information
1. Department of Neurology Hallym University College of Medicine.
- Publication Type:Original Article
- Keywords:
Dopamine transporter;
Parkinson's disease;
6-Hydroxydopamine;
Mazindol, Dopamine;
DOPAC
- MeSH:
3,4-Dihydroxyphenylacetic Acid;
Animals;
Apomorphine;
Autoradiography;
Disease Progression;
Dopamine Plasma Membrane Transport Proteins*;
Dopamine*;
Ligands;
Masks;
Models, Animal;
Oxidopamine*;
Parkinson Disease*;
Rats*;
Rats, Sprague-Dawley;
Substantia Nigra;
Tomography, Emission-Computed, Single-Photon
- From:Journal of the Korean Neurological Association
1998;16(4):536-546
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: Neuroprotective therapy is essential in the management of Parkinson's disease(PD). As symptomatic benefit of a treatment may clinically mask the disease progression, an evaluation of the effect of a neuroprotective therapy should be based on objective measurement of in vivo dopaminergic integrity: Nuclear imaging techniques such as SPECT or PET can visualize dopaminergic system using dopamine transporter ligands and show the promise for this purpose. The objective of this study is to examine the changes of dopamine transporter in the animal model of PD and those correlations with behavioral and biochemical changes. METHODS: We injected 6-hydroxydopamine into the substantia nigra in Sprague-Dawley rats to establish the unilateral PD model, and examined the rotation response after apomorphine injection as a behavioral aspect of the animal model. And we also measured the dopamine and DOPAC level in the striata and the dopamine transporter by [3H]-mazindol autoradiography. RESULTS: We observed that the rats showed turning behavior only after severe reduction of dopamine and DOPAC. There was a strong inverse correlation between rotation behavior and striatal dopamine, DOPAC and dopamine transporter density. There was a positive and strong linear-correlation between dopamine transporter density and dopamine or DOPAC levels. CONCLUSION: Measurement of dopamine transporter gives a good estimate of striatal dopamine level in an animal model of PD. In vivo measurement of dopamine transporter will give an objective information on the integrity of presynaptic nigrostriatal dopaminergic system.
