Effect of Ascorbic Acid Against the Oxidative Stress-Induced Cellular Senescence in Trabecular Meshwork Cells.
10.3341/jkos.2013.54.3.490
- Author:
Jae Woo KIM
1
;
Sun Hee KANG
;
Keun Woo LEE
Author Information
1. Department of Ophthalmology, Catholic University of Daegu School of Medicine, Daegu, Korea. jwkim@cu.ac.kr
- Publication Type:Original Article
- Keywords:
Ascorbic acid;
Oxidative stress;
Senescence;
Trabecular meshwork cells
- MeSH:
Aging;
Ascorbic Acid;
Cell Aging;
Humans;
Hydrogen;
Hydrogen Peroxide;
Nitric Oxide;
Oxidative Stress;
Trabecular Meshwork
- From:Journal of the Korean Ophthalmological Society
2013;54(3):490-495
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: To investigate the effect of L-ascorbic acid (LAA) on oxidative-stress-induced cellular senescence in trabecular meshwork (TM) cells. METHODS: Primarily cultured human TM cells were exposed to 0, 10, or 100 microM hydrogen peroxide for 7 days with or without co-exposure of LAA. Cellular survival and nitrite production were assessed with MTT and Griess assays, respectively. SA-beta-gal staining was performed to quantify cellular senescence. RESULTS: Hydrogen peroxide decreased cellular survival, accompanied by decreased nitric oxide (NO) production. These decreases of cellular survival and NO production were abolished by co-exposure of 100 microM LAA. Analysis of SA-beta-gal staining revealed that LAA inhibited hydrogen peroxide-induced cellular senescence by 6.8% (p < 0.05). CONCLUSIONS: LAA may have a protective effect against the oxidative-stress-induced cellular senescence in TM cells.
