Serotonin transporter mRNA expression in the dorsal raphe nucleus of a tumor bearing mouse.
- Author:
Jeong Won JAHNG
1
;
Si Ho CHOI
;
Mi Joo CHA
;
Nam Youl KIM
;
Soon Jeong HWANG
;
Jong Ho LEE
Author Information
1. Department of Pharmacology, BK21 Project for Medical Science, Yonsei University College of Medicine, Seoul, Korea.
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
depression;
gene expression;
in situ hybridization;
leptin;
serotonin;
tumor
- MeSH:
Animals;
Body Weight;
Carcinoma, Squamous Cell/*metabolism/pathology;
DNA, Complementary;
*Gene Expression Regulation, Neoplastic;
Humans;
Leptin/blood;
Male;
Membrane Glycoproteins/*genetics/metabolism;
Membrane Transport Proteins/*genetics/metabolism;
Mice;
Mice, Inbred BALB C;
Mice, Nude;
Mouth Neoplasms/*metabolism/pathology;
Nerve Tissue Proteins/*genetics/metabolism;
RNA, Messenger/*metabolism;
Radioimmunoassay;
Raphe Nuclei/*metabolism;
Research Support, Non-U.S. Gov't;
Serotonin/metabolism
- From:Experimental & Molecular Medicine
2005;37(1):65-69
- CountryRepublic of Korea
- Language:English
-
Abstract:
This study was conducted to determine if an oral squamous cell carcinoma alters mRNA expression of serotonin transporter (5-HTT) in the central nervous system. KB cell line derived from a human oral squamous cell carcinoma was inoculated into nude mice, and mRNA expression level of 5-HTT in the dorsal raphe nucleus (DRN) was examined by in situ hybridization when the tumor mass reached to -10% of total body weight. Plasma leptin levels were determined by radioimmunoassay method using a commercial kit. 5-HTT mRNA level was significantly decreased in the DRN of tumor bearing mice, compared to the age-matching non-tumor control. Plasma leptin level decreased concomitantly in tumor bearing mice. These results suggest that oral carcinoma may suppress 5-HTT gene expression in the central nervous system, perhaps in relation with decreased plasma leptin level.
