Comparison of the Rate of Detection of Immunoglobulin Heavy Chain Gene Rearrangement by Fluoresecence In Situ Hybridization Probes in Multiple Myeloma.
10.3343/kjlm.2006.26.5.317
- Author:
Hye Yoon CHUNG
1
;
Cha Ja SEE
;
Jung Eun CHOI
;
Hyun Jung MIN
;
Han Ik CHO
;
Dong Soon LEE
Author Information
1. Department of Laboratory Medicine, College of Medicine, Seoul National University, Seoul, Korea. soonlee@plaza.snu.ac.kr
- Publication Type:Original Article
- Keywords:
Immunoglobulin heavy chain gene rearrangement;
FISH;
Multiple myeloma
- MeSH:
Bone Marrow;
Chungcheongnam-do;
Diagnosis;
Fluorescence;
Gene Rearrangement*;
Humans;
Immunoglobulin Heavy Chains*;
Immunoglobulins*;
In Situ Hybridization*;
Multiple Myeloma*;
Seoul
- From:The Korean Journal of Laboratory Medicine
2006;26(5):317-322
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: Immunoglobulin heavy chain (IgH) gene rearrangement, which is frequently observed in multiple myeloma, can now be detected easily by using a fluorescence in situ hybridization (FISH) method. The aim of this study was to determine the detection rate and compare the utility of the three most commonly used probes: IGH/CCND1 dual color, dual fusion probe; IGH/BCL2 dual color, dual fusion probe; and IGH dual color break apart rearrangement probe; all from Vysis Products (Downers Grove, IL, USA). METHODS: From October 1994 to July 2003, 99 patients were diagnosed as multiple myeloma at Seoul National University Hospital, Asan Medical Center and Gachon University Gil hospital. We applied the three different probes of IgH FISH on bone marrow specimens from the 99 Korean patients with multiple myeloma to detect IgH gene rearrangement. RESULTS: Forty-one (41.4%) of the 99 patients had IgH gene rearrangement. Of those 41 patients, 23 (56.1%) showed positive to all three probes, but the remaining 18 (43.9%) showed a discrepancy between the three probes: 13 (72.2%) of the 18 patients were only positive to the IGH dual color break apart rearrangement probe and the detection rate was 39.6% on the average. CONCLUSIONS: These results demonstrate that IGH dual color break apart rearrangement probe is superior to the other two probes in qualitative and quantitative ways. Thus, we recommend IGH dual color break apart rearrangement probe for the diagnosis and monitoring of multiple myeloma.
