Protease-Activated Receptor-2 Is Associated with Terminal Differentiation of Epidermis and Eccrine Sweat Glands.
- Author:
Yong Sup SHIN
1
;
Hyung Won KIM
;
Chang Deok KIM
;
Hyun Woo KIM
;
Jin Woon PARK
;
Sunggyun JUNG
;
Jeung Hoon LEE
;
Young Kwon KO
;
Young Ho LEE
Author Information
- Publication Type:Original Article
- Keywords: Eccrine sweat glands; Epidermis; Keratinocytes; Terminal differentiation
- MeSH: Blotting, Western; Bony Callus; Calcium; Carcinoma, Squamous Cell; Cell Line; Epidermis*; Homeostasis; Humans; Inflammation; Keratinocytes; Melanosomes; Pain Perception; Receptor, PAR-2; Skin; Sweat Glands*; Sweat*; Syringoma
- From:Annals of Dermatology 2015;27(4):364-370
- CountryRepublic of Korea
- Language:English
- Abstract: BACKGROUND: Protease-activated receptor 2 (PAR-2) participates in various biological activities, including the regulation of epidermal barrier homeostasis, inflammation, pain perception, and melanosome transfer in the skin. OBJECTIVE: To evaluate the basic physiological role of PAR-2 in skin. METHODS: We investigated PAR-2 expression in human epidermis, skin tumors, and cultured epidermal cells using western blot and immunohistochemical analysis. Additionally, we examined the effect of the PAR-2 agonist, SLIGRL-NH2, on cultured keratinocytes. RESULTS: Strong PAR-2 immunoreactivity was observed in the granular layer of normal human skin and the acrosyringium of the eccrine sweat glands. In contrast, weak PAR-2 immunoreactivity was seen in the granular layer of callused skin and in the duct and gland cells of the eccrine sweat glands. Interestingly, PAR-2 immunoreactivity was very weak or absent in the tumor cells of squamous cell carcinoma (SCC) and syringoma. PAR-2 was detected in primary keratinocytes and SV-40T-transformed human epidermal keratinocytes (SV-HEKs), an immortalized keratinocyte cell line, but not in SCC12 cells. SV-HEKs that were fully differentiated following calcium treatment displayed higher PAR-2 expression than undifferentiated SV-HEKs. Treatment of cultured SV-HEKs with PAR-2 agonist increased loricrin and filaggrin expression, a terminal differentiation marker. CONCLUSION: Our data suggest that PAR-2 is associated with terminal differentiation of epidermis and eccrine sweat glands.
