Magnesium Ascorbyl Phosphate Regulates the Expression of Inflammatory Biomarkers in Cultured Sebocytes.
- Author:
Weon Ju LEE
1
;
Sang Lim KIM
;
Yoon Seok CHOE
;
Yong Hyun JANG
;
Seok Jong LEE
;
Do Won KIM
Author Information
- Publication Type:Original Article
- Keywords: Acne vulgaris; Ascorbate-2-phosphate; Cytokines; Lipopolysaccharides; Sebocytes
- MeSH: Acne Vulgaris; Ascorbic Acid; Biomarkers*; Blotting, Western; Cytokines; Enzyme-Linked Immunosorbent Assay; Lipid Peroxidation; Lipopolysaccharides; Magnesium*; Matrix Metalloproteinases; Peptides; Skin; Toll-Like Receptors
- From:Annals of Dermatology 2015;27(4):376-382
- CountryRepublic of Korea
- Language:English
- Abstract: BACKGROUND: Acne is an inflammatory skin disorder caused by inflammatory biomarkers. Magnesium ascorbyl phosphate (MAP) is a stable precursor of vitamin C. It achieves a constant delivery of vitamin C into the skin and has antioxidative effects. OBJECTIVE: We performed this study to evaluate the effect of MAP on the expression of inflammatory biomarkers in cultured sebocytes. METHODS: Reverse transcription-polymerase chain reaction (RT-PCR) and enzyme-linked immunosorbent assay were performed for inflammatory cytokines and matrix metalloproteinases (MMPs) before and after treatment of cultured sebocytes with MAP (10(-2) M), lipopolysaccharide (LPS) (5 microg/ml) and a combination of MAP and LPS. RT-PCR and western blotting were also performed for antimicrobial peptides (AMPs) and Toll-like receptor (TLR)-4 before and after treatment of cultured sebocytes with MAP, LPS, and a combination of MAP and LPS. Quantification of lipid peroxidation was also conducted. RESULTS: The increased expression of inflammatory cytokines after treatment of cultured sebocytes with LPS was decreased after treatment with MAP. MMPs, AMPs, and TLR-4 were decreased after treatment of cultured sebocytes with MAP and a combination of MAP and LPS, and increased after treatment of cultured sebocytes with LPS alone. Lipid peroxidation was significantly decreased after treatment of cultured sebocytes with MAP and a combination of MAP and LPS. MAP decreased the increased lipid peroxidation after treatment of cultured sebocytes with LPS. CONCLUSION: MAP may be an effective alternative agent to improve inflammatory reactions in acne.
