Bone Marrow T Cells are Superior to Splenic T Cells to Induce Chimeric Conversion After Non-Myeloablative Bone Marrow Transplantation.
10.3904/kjim.2009.24.3.252
- Author:
Hyun Sil PARK
1
;
Seok Goo CHO
;
Min Jung PARK
;
So Youn MIN
;
Hong Seok CHANG
;
Hee Je KIM
;
Seok LEE
;
Chang Ki MIN
;
Jong Wook LEE
;
Woo Sung MIN
;
Chun Choo KIM
;
Ho Youn KIM
Author Information
1. Rheumatism Research Center, Catholic Research Institutes of Medical Science, The Catholic University of Korea College of Medicine, Seoul, Korea.
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
Chimerism, mixed;
Infusion, donor lymphocyte;
T-cells, bone marrow
- MeSH:
Animals;
Bone Marrow Cells/*physiology;
*Bone Marrow Transplantation;
Female;
Graft vs Host Disease/prevention & control;
*Lymphocyte Transfusion;
Mice;
Mice, Inbred BALB C;
Mice, Inbred C57BL;
Spleen/*cytology;
T-Lymphocytes/*physiology;
Tissue Donors;
Transplantation Chimera;
Transplantation, Homologous
- From:The Korean Journal of Internal Medicine
2009;24(3):252-262
- CountryRepublic of Korea
- Language:English
-
Abstract:
BACKGROUND/AIMS: The bone marrow functions not only as the primary B-lymphocyte-producing organ but also as a secondary lymphoid organ for CD4 and CD8 cell responses and a site of preferential homing and persistence for memory T cells. Bone marrow T (BM-T) cells are distinguished from peripheral blood T cells by surface phenotype, cytokine secretion profile, and immune functions. In this study, we evaluated the alloreactive potential of donor lymphocyte infusion (DLI) using BM-T cells in mixed chimerism compared to that using spleen T (SP-T) cells. METHODS: Cells were prepared using established procedures. BM-T cells were obtained as a by-product of T-cell depletion in BM grafting and then cryopreserved for subsequent DLI. We performed DLI using BM-T cells in allogeneic mixed chimera mice on post-BMT day 21. RESULTS: When the same dose of T cells, 5-10x10(5) (Thy1.2+), fractionated from BM and spleen were administered into mixed chimeras, the BM-T group showed complete chimeric conversion, with self-limited graft-versus-host disease (GVHD) and no pathological changes. However, the SP-T group showed persistent mixed chimerism, with pathological signs of GVHD in the liver and intestine. CONCLUSIONS: Our results suggest that DLI using BM-T cells, even in small numbers, is more potent at inducing chimeric conversion in mixed chimerism than DLI using SP-T cells. Further study is needed to determine whether cryopreserved BM-T cells are an effective cell source for DLI to consolidate donor-dominant chimerism in clinical practice without concerns about GVHD.
