Protein Expression of the Human Norovirus Capsid Gene using the Baculovirus Expression System.
10.4167/jbv.2011.41.3.183
- Author:
Ji Young JIN
1
;
Chul Jong PARK
;
Seung Won PARK
;
Soon Young PAIK
Author Information
1. Department of Microbiology, College of Medicine, The Catholic University, Seoul, Korea. paik@catholic.ac.kr
- Publication Type:Original Article
- Keywords:
Human norovirus;
Baculovirus expression system
- MeSH:
Baculoviridae;
Capsid;
Capsid Proteins;
Gastroenteritis;
Genome;
Humans;
Norovirus;
Open Reading Frames
- From:Journal of Bacteriology and Virology
2011;41(3):183-187
- CountryRepublic of Korea
- Language:English
-
Abstract:
Human norovirus (HuNoV) is the major etiological agent of nonbacterial gastroenteritis worldwide. However, due to the absence of a rapid and sensitive diagnostic system, detection and monitoring have been limited. The HuNoV genome is composed of three open reading frames (ORFs). And major capsid protein, ORF2, is designated as a viral protein 1 (VP1). In this study, the baculovirus expression system was used for expression of the HuNoV capsid protein, VP1. Recombinant baculoviruses can be used as potent tools in HuNoV studies.
