A Study on Expression of Ki-67 Antigen in Melanocytic Skin Lesions.
- Author:
Tae Jin CHOI
;
Doe Kywn ROH
;
Dong Won LEE
;
Si Yong KIM
;
Baik Kee CHO
;
Chang Suk KANG
- Publication Type:Original Article
- Keywords:
Ki-67;
MIB1;
Melanocytic nevus;
Malignant melanoma
- MeSH:
Antibodies;
Antibodies, Monoclonal;
Cell Proliferation;
Diagnosis;
Humans;
Ki-67 Antigen*;
Melanoma;
Nevus, Pigmented;
Prognosis;
Retrospective Studies;
Skin*
- From:Korean Journal of Dermatology
1998;36(2):217-223
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: Evaluation of the cell proliferation activity of neoplasm is useful in making a diagnosis and prognosis. Immunohistochemical staining using MIB1 monoclonal antibodies has recently been demonstrated to assess tumor cell proliferation activity easily, because it can be performed on paraffin-embedded specimens and is available in a retrospective study. OBJECTIVE: The purpose of this study to investigate (a) the use of Ki-67 antibodies in distinguising between benign melanocytic nevus and malignant melanoma, and (b) the correlation between the Ki-67 labelling index(positive number per 100cells) and histological parameters such as the Breslow index, Clarks level, mitotic rate(mitoses per 100cells) in malignant melanoma. METHOD: A total of 37 formalin-fixed, paraffin-embedded melanocytic skin lesions(benign melanocytic nevus 21; malignant melanoma 16) were stained with MIB1 using the one hour method. The values of the Ki-67 labelling index were measured in all the lesions. For each case of malignant melanoma, the Breslow index, Clarks level and mitotic rate were quantitatively evaluated. RESULT: The Ki-67 labelling index averaged 2.6 +1.4 in benign melanocytic nevus and 22.4+ 6.2 in malignant melanoma. In malignant melanoma, the values of the Ki-67 labelling index were significantly correlated with Breslow index(r=0.764), Clark's level(r=0.885) and mitotic rate(r=0.754). CONCLUSION: Our findings suggest that Ki-67 may be a useful tool in distinguising between benign melanocytic nevus and malignant melanoma. According as Ki-67 labelling index correlates with well-established prognostic parameters(Breslow index, Clark's level, mitotic rate), it may therefore carry additional prognostic value for patients with malignant melanoma.
