Immunohistochemical Staining in the Differential Diagnosis of Trichoblastoma and Basal cell Carcinoma.
- Author:
Kwang Hyun CHO
1
;
Kwang Ho HAN
;
Sang Eun MOON
;
Jai Il YOUN
;
Hee Chul EUN
;
Chul Woo KIM
Author Information
1. Department of Dermatology, Seoul National University College of Medicine, Seoul, Korea.
- Publication Type:Original Article
- Keywords:
Trichoblastoma;
Basal cell carcinoma;
Immunohistochemistry
- MeSH:
Antibodies, Monoclonal;
Biotin;
Carcinoma, Basal Cell*;
Cytoplasm;
Diagnosis;
Diagnosis, Differential*;
Immunohistochemistry;
Paraffin;
Peroxidase;
Skin
- From:Korean Journal of Dermatology
1999;37(10):1423-1429
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: Trichoblastoma is a benign skin tumor with follicular differentiation which sometimes is difficult to distinguish histologically from basal cell carcinoma (BCC). OBJECTIVE: Our purpose is to investigate the usefulness of the immunostains in the differential diagnosis of trichoblastoma and BCC. METHODS: The authors analyzed 11 trichoblastoma, 8 keratotic BCCs, 9 nodular BCCs, 6 borderline cases. The monoclonal antibodies to bcl-2, CD34, Ki-67, p53 were applied to formalin-fixed paraffin sections in all cases, using a labelled streptoavidin biotin peroxidase complex method. RESULTS: Most nodular BCCs demonstrated diffuse cytoplasm labelling for bcl-2. In contrast, 'peripheral' bcl-2 staining of trichoblastomas was noted in 9 of 11. Keratotic BCCs demonstrated mixed staining pattern for bcl-2. CD34 staining failed to discriminate between trichoblastomas and BCCs. In BCC, Ki-67 positive cells were mainly distributed in the perphery of tumors. CONCLUSION: Immunostain using monoclonal antibodies to bcl-2, CD34, Ki-67, p53 can not differentiate between trichoblastomas and BCCs. But it could be an adjunctive method to establish the definite diagnosis of trichoblastomas and BCCs.
