Neurogenic potentials of human amniotic fluid-derived stem cells according to expression levels of stem cell markers and ingredients of induction medium.
- Author:
Eun Hye LIM
1
;
Jung Ah CHO
;
Ho PARK
;
Tae Jong SONG
;
Woo Young KIM
;
Kye Hyun KIM
;
Kyo Won LEE
Author Information
- Publication Type:Original Article
- Keywords: Stem cells; Amniotic fluid; Neurogenesis
- MeSH: Amniotic Fluid; Female; Fluorescent Antibody Technique; Gene Expression; Humans; Neurogenesis; Stem Cells*; Tretinoin; Up-Regulation
- From:Journal of Genetic Medicine 2015;12(1):31-37
- CountryRepublic of Korea
- Language:English
- Abstract: PURPOSE: We investigated the neurogenic potentials of amniotic fluid-derived stem cells (AFSCs) according to the expression levels of stem cell markers and ingredients in the neural induction media. MATERIALS AND METHODS: Four samples of AFSCs with different levels of Oct-4 and c-kit expression were differentiated neurally, using three kinds of induction media containing retinoic acid (RA) and/or a mixture of 3-isobutyl-1-methylxanthine/indomethacin/insulin (neuromix), and examined by immunofluorescence and reverse transcription-polymerase chain reaction (RT-PCR) for their expression of neurospecific markers. RESULTS: The cells in neuromix-containing media displayed small nuclei and long processes that were characteristic of neural cells. RT-PCR analysis revealed that the number of neural markers showing upregulation was greater in cells cultured in the neuromix-containing media than in those cultured in RA-only medium. Neurospecific gene expression was also higher in Oct-4 and c-kit double-positive cells than in c-kit-low or -negative cells. CONCLUSION: The stem cell marker c-kit (rather than Oct-4) and the ingredient neuromix (rather than RA) exert greater effects on neurogenesis of AFSCs.
