Constituents Released from Streptococcus mutans Attenuate Arecoline-mediated Cytotoxicity in HGF Cells by Altering Intracellular Ca2+ Signaling.
- Author:
Munkhsoyol ERKHEMBAATAR
1
;
Hyuncheol OH
;
Min Seuk KIM
Author Information
1. Department of Oral Physiology, College of Dentistry, Wonkwang University, Iksan 570-749, Republic of Korea. happy1487@wku.ac.kr
- Publication Type:Original Article
- Keywords:
Streptococcus mutans;
arecoline;
human gingival fibroblast;
Ca2+ signaling;
cytotoxicity
- MeSH:
Areca;
Arecoline;
Biofilms;
Epithelial Cells;
Fibroblasts;
Gingivitis;
Homeostasis;
Humans;
Metabolism;
Microbiota;
Mouth;
Nuts;
Streptococcus mutans*;
Tooth
- From:International Journal of Oral Biology
2014;39(1):41-47
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
Streptococcus mutans (S. mutans) is a facultative anaerobic bacterium mainly found in the oral cavity and is known to contribute to tooth decay and gingivitis. Recent studies on intestinal microbiota have revealed that microorganisms forming a biofilm play important roles in maintaining tissue homeostasis through their own metabolism. However, the physiological roles of oral microorganisms such as S. mutans are still unclear. In our current study, we identified that constituents released from S. mutans (CR) reduce arecoline-mediated cytotoxicity without producing toxic effects themselves. Arecoline, as a major alkaloid of areca nut, is known to mediate cytotoxicity on oral epithelial cells and induces a sustained intracellular Ca2+ ([Ca2+]i) increase that is cytotoxic. The exposure of human gingival fibroblast (HGF) cells to CR not only inhibited the sustained [Ca2+]i increase but also the initial [Ca2+]i elevation. In contrast, CR had no effects on the gene regulation mediated by arecoline. These results demonstrate that S. mutans has physiological role in reducing cytotoxicity in HGF cells and may be considered a novel pharmaceutical candidate.
