The Effect of Intra-portal Infusion of Glucose-insulin-potassium (GIK) Solution on the Energy Metabolism during Cold Preservation in a Small-animal Model for Liver Transplantation.
- Author:
Tae Yong HA
1
;
Shin HWANG
;
Sung Gyu LEE
;
He Nam HONG
;
Chul Soo AHN
;
Ki Hun KIM
;
Deok Bog MOON
;
Gi Won SONG
;
Dong Hwan JUNG
;
Je Ho RYU
;
Nam Kyu CHOI
;
Kwan Woo KIM
;
Kyoung Hun KO
;
Guang LI
Author Information
1. Department of Surgery, Division of Liver Transplantation and Hepatobiliary Surgery, University of Ulsan College of Medicine, Asan Medical Center, Seoul, Korea. shwang@amc.seoul.kr
- Publication Type:Original Article
- Keywords:
Liver transplantation;
Glycogen;
Energy metabolism;
Cold preservation
- MeSH:
Adenosine Triphosphate;
Animals;
Apoptosis;
Biopsy;
Cold Temperature;
Energy Metabolism;
Glucose;
Glycogen;
Humans;
Insulin;
Liver;
Liver Transplantation;
Malnutrition;
Mannitol;
Perfusion;
Potassium;
Potassium Chloride;
Procaine;
Rats;
Transplants
- From:The Journal of the Korean Society for Transplantation
2008;22(1):41-48
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: The effect of intra-portal infusion of glucose-insulin-potassium (GIK) solution on the energy metabolism during cold preservation was investigated using a small-animal liver transplantation model. METHODS: Fifteen white rats were divided into 3 groups: the group A (feeding group) were fed normally before experiment. The group B (fasting group) and group C (GIK group) were fasted from 3 days before experiment, by which acute nutritional deficiency state was induced. In group A and B, the whole liver was procured after intra-portal perfusion of HTK solution and serial liver biopsies were performed during the cold preservation period with 4degrees C HTK solution. In group C, intra-portal GIK solution infusion for 1 hour preceded liver graft harvest. From the liver tissues, the relative intracellular glycogen contents and the ATP concentration were measured. RESULTS: Relative glycogen contents in group A were 100% at 0 h, 64.6% at 2 h, 54.9% at 4 h, and 16.2% at 8 h; 10.3%, 8.3%, 4.9% and 0%, respectively in group B; 109.2%, 96.9%, 54.2% and 9.7%, respectively in group C. There was a temporary supercharge of ATP level in group C only at 0 h. Apoptosis was less expressed in group C comparing with group A and B. CONCLUSION: Rapid intra- portal infusion of GIK solution could make intrahepatic glycogen content fully restored to the normal level. Considering that intracellular glycogen is the main energy source during immediate post-transplant period, its restoration may contribute to improvement of post-transplant graft function.