Sensitivity of CD95-induced apoptosis in different proliferative status of human retinal pigment epithelial cells.
- Author:
Jin Hee CHANG
1
;
Se Woong KANG
;
Don Il HAM
Author Information
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords: Apoptosis; retinal pigment epithelial cells; proliferation
- MeSH: Antigens, CD95/*pharmacology; Apoptosis/*physiology; Cells, Cultured; Human; Pigment Epithelium of Eye/cytology/*drug effects/*physiology; Sensitivity and Specificity
- From:Korean Journal of Ophthalmology 2001;15(2):74-80
- CountryRepublic of Korea
- Language:English
- Abstract: It is known that CD95 (APO-1/Fas) is expressed on the cell surface, and apoptotic cell death can be induced by the CD95 ligation in the cultured, proliferating human retinal pigment epithelial (RPE) cells. However, little is known about CD95 on the non-proliferating RPE cells. In this study, human RPE cells were cultured up to 4 weeks after they reached the confluence, to simulate the non-proliferating RPE cells in situ. There was no significant difference in CD95 expression on the cell surface between the predominantly proliferating, preconfluent cells and predominantly non-proliferating, postconfluent cells in flow cytometric assays. However, unlike proliferating cells, no cellular death occurred in the predominantly non-proliferating cells after the treatment of agonistic anti-CD95 antibody with cycloheximide, pretreated with interferon-gamma. Our results suggest that the CD95/CD95L system probably plays a physiologic role in vivo to remove the abnormal, proliferating RPE cells, and factors other than the surface expression of CD95 may determine the sensitivity to the CD95 signals.
