Significance of Tumor Angiogenesis and p53 Protein Expression in Pancreatic Adenocarcinoma.
- Author:
Sang Soon KIM
1
;
Min Chan KIM
;
Sook Hee HONG
Author Information
1. Department of Surgery, Dong-A University College of Medicine, Busan, Korea.
- Publication Type:Original Article
- Keywords:
Pancreatic adenocarcinoma;
Tumor angiogenesis;
p53 protein;
Prognosis;
Recurrence
- MeSH:
Adenocarcinoma*;
Antibodies;
Humans;
Microvessels;
Prognosis;
Recurrence;
Survival Rate
- From:Journal of the Korean Surgical Society
2001;61(1):86-94
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: This study was designed to examine the microvessel count and p53 protein expression level in pancreatic adenocarcinoma patients and to determine how they correlate with the clinicopathologic factors and prognosis. In addition, the relationship between angiogenesis and p53 protein expression in pancreatic adenocarcinoma patients was investigated. METHODS: Paraffin-embedded tumor tissues from 30 patients with pancreatic adenocarcinomas that were completely removed by a radical resection were retrieved and analyzed. The vessels were immunostained with anti-factor VIII polyclonal antibodies, and 5 areas with the most discrete microvessels were counted under a 200 field. The mean was calculated, which was defined as the angiogenesis score (AS). p53 protein expression was detected by the immunohistochemical stain method. RESULTS: The mean AS was 37.7 13.6. There were no significant differences in sex, age, tumor size, histologic grade, T category (depth of invasion), N category (lymph node metastasis), pathologic stage and recurrence between the high and low AS group. The p53 protein expression rate was 73.3%, which did not correlate with the clinicopathologic factors and AS. However, recurrence was significantly related to the histologic grade and pathologic stage (p=0.040 and p=0.029). Among the various clinicopathologic factors, the N category (lymph node metastasis), the AS and recurrence (p=0.025, p=0.029 and p=0.003) were statistically significant according to a univariate survival analysis. In theCox regression analysis, the N category (lymph node metastasis) and recurrence were independent prognostic factors (odds ratio=4.344, 4.263). CONCLUSION: Although the survival rate in the high AS group was significantly lower than that in low AS group (p=0.029), the angiogenesis assessed by the microvessel count using immunohistochemical staining was not an independent prognostic factor in the Cox regression analysis.
