A New Rat Model of Cisplatin-induced Neuropathic Pain.
10.3344/kjp.2015.28.4.236
- Author:
Hai LIN
1
;
Bong Ha HEO
;
Myung Ha YOON
Author Information
1. Department of Anesthesiology and Pain Medicine, Chonnam National University Medical School, Gwangju, Korea. mhyoon@chonnam.ac.kr
- Publication Type:Original Article
- Keywords:
Chemotherapy;
Cisplatin;
Models;
Neuropathy;
Pain;
Rats
- MeSH:
Acetone;
Animals;
Body Weight;
Cisplatin;
Drug Therapy;
Female;
Hot Temperature;
Humans;
Hyperalgesia;
Models, Animal*;
Neoplasm Metastasis;
Neuralgia*;
Peripheral Nervous System Diseases;
Rats*;
Rats, Sprague-Dawley
- From:The Korean Journal of Pain
2015;28(4):236-243
- CountryRepublic of Korea
- Language:English
-
Abstract:
BACKGROUND: Chemotherapy-induced peripheral neuropathy is a major side effect of anti-cancer drugs, and our knowledge of its mechanisms is lacking. Several models for chemotherapy-induced neuropathy have been introduced. However, the outcomes of these models differ significantly among laboratories. Our object was to create a model of chemotherapy-induced neuropathy in rats with cancer. METHODS: Female Sprague-Dawley rats were used. Mammary rat metastasis tumor (MRMT-1) cells were implanted subcutaneously in rats. Chemotherapy-induced peripheral neuropathy was induced by injection of cisplatin once a day for four days. The responses to mechanical and thermal stimuli were examined using von Frey filaments, acetone, and radiant heat. RESULTS: Cisplatin (2 mg/kg/day) produced mechanical allodynia, while it did not induce cold allodynia or thermal hyperalgesia. This dose of cisplatin could work successfully against cancer. Body weight loss was not observed in cisplatin-treated rats, nor were other abnormal behaviors noted in the same rats. CONCLUSIONS: Repeated injection of intraperitoneal cisplatin induced peripheral neuropathic pain in rats. Thus, this type of rat model has broad applicability in studies related to searching for the mechanism of cisplatin-induced mechanical allodynia and agents for the treatment of neuropathic pain.
