Effect of Trypan Blue on the Survival of Cultured Trabecular Meshwork Cells.
10.3341/jkos.2017.58.3.327
- Author:
Hye Ri KANG
1
;
Seok Jin HWANG
;
Jae Woo KIM
Author Information
1. Department of Ophthalmology, Catholic University of Daegu School of Medicine, Daegu, Korea. jwkim@cu.ac.kr
- Publication Type:Original Article
- Keywords:
Apoptosis;
Flow cytometry;
Survival;
Trabecular meshwork cell;
Trypan blue
- MeSH:
Apoptosis;
Cataract;
Cell Survival;
Flow Cytometry;
Humans;
Trabecular Meshwork*;
Trypan Blue*
- From:Journal of the Korean Ophthalmological Society
2017;58(3):327-332
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: To evaluate the effects of trypan blue (TB) on the survival of cultured human trabecular meshwork cells (HTMCs). METHODS: Primarily cultured HTMCs were exposed to 0.05, 0.10 or 0.50% TB for 1, 5 or 30 min. Cellular survival was assessed using the MTT assay and degree of apoptosis was analyzed with flow cytometry using annexin-V/propidium iodide double staining. RESULTS: Long-term exposure or high concentration of TB decreased the survival of HTMCs (p < 0.05). In flow cytometric analysis, exposure to 0.50% TB for 30 min increased the degree of apoptosis (p < 0.05). Commercial TB decreased cell survival after exposure for 5 min and increased the degree of apoptosis after exposure for 30 min (p < 0.05). CONCLUSIONS: TB may cause cellular damage of cultured HTMCs and apoptosis could be the underlying mechanism. In TB-assisted cataract surgery, TB should be used for the shortest time possible and removed completely.
