Appearance of Polymorphonuclear Leukocytes Following Corneal Scratching Wound by Impression Cytology.
- Author:
Jin Sang KIM
1
;
Myung Kyoo KO
Author Information
1. Department of Ophthalmology Pyung-dong, 108, Chongno-gu, Seoul, Korea.
- Publication Type:Original Article
- Keywords:
Cornea;
Impression cytology;
Polymorphonuclear leukocyte;
Scratching wound
- MeSH:
Cornea;
Eosine Yellowish-(YS);
Epithelial Cells;
Epithelium, Corneal;
Hematoxylin;
Needles;
Neutrophils*;
Tetracaine;
Wounds and Injuries*
- From:Journal of the Korean Ophthalmological Society
1997;38(10):1729-1736
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
The linear scratching wound was made gently on the corneal epithelium of rabbit with 21 gauge needle under an operating microscope. Impression cytology was performed at 30 minutes, 1, 2, 3 and 4 hour and 1, 2, 3 and 4 day after 0.5% tetracaine drops under an operating microscope. The filter paper was stained with hematoxylin and eosin. At 30 minute post-scratching, a few polymorphonuclear leukocytes appeared on the scratched cornea at 3 eyes (30%). At 3 and 4 hour, numerous polymorphonuclear leukocytes with corneal epithelial cells appeared on the scratched conea. By 3 day, no inflammatory cells were shown on the filter paper in all eyes. These findings suggest that the polymorphonuclear leukocytes could infiltrate on the corneal lesion at 30 minute post-scratching and the inflammatory cells might act even on the minute corneal lesion such as corneal erosion.
