Reactive Oxygen Species are Involved in Y-27632-induced Neurite Outgrowth in PC12 Cells.
10.11620/IJOB.2016.41.4.231
- Author:
So Yeong PARK
1
;
Seong Ah MOON
;
Jeong Mi AN
;
Du sik KIM
;
Jeong Taeg SEO
Author Information
1. Department of Oral Biology, Yonsei University College of Dentistry, Seoul 120-752, Korea. jeong@yuhs.ac
- Publication Type:Original Article
- Keywords:
Y-27632;
ROCK;
PC12 cells;
neurite outgrowth
- MeSH:
Acetylcysteine;
Animals;
Neurites*;
Neurons;
PC12 Cells*;
Phosphotransferases;
Reactive Oxygen Species*
- From:International Journal of Oral Biology
2016;41(4):231-236
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
Inhibition of Rho-associated coiled coil-containing kinase (ROCK) has been reported to promote differentiation of neuronal cells. Here, we examined the effect of Y-27632, a ROCK inhibitor, on the outgrowth of neurites in PC12 cells. Y-27632 caused a rapid induction of neurite outgrowth in PC12 cells in a time-dependent manner. The neurite outgrowth, triggered by Y-27632, was accompanied by Rac1 activation, and was attenuated by Rac1 inhibitor NSC23766, in a concentration-dependent manner. Y-27632 also induced an increase in the production of reactive oxygen species (ROS). Pretreatment with N-acetylcysteine, an ROS scavenger, inhibited the ROS generation and neurite outgrowth in response to Y-27632. These results indicate that the activation of Rac1 and the generation of ROS contribute to the neurite outgrowth triggered by Y-27632 in PC12 cells.
