Expression of Inflammasome Complex Following Various Oral Bacterial Infection in THP-1 Cells.
10.11620/IJOB.2016.41.4.237
- Author:
Hee Sam NA
1
;
Su Mi KIM
;
Yu Ri SONG
;
Seyeon KIM
;
Jin CHUNG
Author Information
1. Department of Oral Microbiology, School of Dentistry, Pusan National University, Yangsan 626-870, Korea. jchung@pusan.ac.kr
- Publication Type:Original Article
- Keywords:
Oral bacteria;
inflammasome;
IL-1 beta;
real-time PCR
- MeSH:
Bacteria;
Bacterial Infections*;
Immunity, Innate;
Inflammasomes*;
Interleukin-1beta;
Real-Time Polymerase Chain Reaction;
RNA, Messenger
- From:International Journal of Oral Biology
2016;41(4):237-242
- CountryRepublic of Korea
- Language:English
-
Abstract:
Interleukin-1b (IL-1β), a proinflammatory cytokine, regulates the innate immune responses against bacterial infection. Mature IL-1β is produced from pro-IL-1β by activated caspase-1, which in turn is activated by the inflammasome complex formation. In this study, we compared the inflammasome mRNA expression induced by S. sanguinis, S. oralis, F. nucleatum and P. intermedia. Among the tested bacteria, S. sanguinis induced the highest IL-1β secretion. S. oralis, F. nucleatum and P. intermedia induced very weak IL-1β secretion. S. sanguinis mostly induced the NLRP3 mRNA expressions. Although F. nucleatum did not induce high IL-1β secretion, it induced high expression levels of AIM2, NLRP2, and NLRP3. No specific inflammasomes were induced by S. oralis and P.intermedia. Studying the inflammasome complex activation induced by oral bacteria may thus enhance our understanding of the pathogenesis of oral diseases.
