Chromosomal gains and losses in primary ovarian carcinomas by comparative genomic hybridization.
- Author:
Soo Hun CHO
1
;
Mee Hye KIM
;
Nak Woo LEE
;
Young Tae KIM
;
Kyu Wan LEE
Author Information
1. Department of Obstetrics and Gynecology, College of Medicine, Korea University, Korea.
- Publication Type:Original Article
- Keywords:
ovarian cancer;
comparative genomic hybridization;
FISH;
chromosomal change
- MeSH:
Base Sequence;
Carcinogenesis;
Comparative Genomic Hybridization*;
DNA;
Genes, myc;
Genes, ras;
Humans;
Mass Screening;
Oncogenes;
Ovarian Neoplasms
- From:Korean Journal of Obstetrics and Gynecology
2003;46(1):38-43
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
OBJECTIVE: Comparative genomic hybridization was performed to evaluate DNA sequence copy number changes in human ovarian carcinomas from paraffin-embedded tissue blocks. PATIENTS AND METHODS: DNA from 20 cases of primary ovarian carcinomas underwent comparative genomic hybridization to evaluate the extent of genetic gains or losses in a test sample. RESULTS: In thirteen cases of 20 samples, varying degree of genetic imbalances was observed. Of the remaining 7 cases, two revealed normal, five failed to yield a result. Most common genetic imbalances are 8q22.2-q24 site amplification and 12p site amplification, where c-myc gene and k-ras gene respectively are included. Second most common site of genetic imbalance is 7p21-pter site deletion. CONCLUSION: Our results have shown many chromosomal alterations in human ovarian carcinomas, and these sites are known previously as oncogene or tumor-suppression gene, and some sites are not known specific cancer associated sites. Our data can be useful for screening chromosomal changes and molecular mechanism of human ovarian carcinogenesis.
