Molecular Genetic Analysis of Cis-AB Blood Group in Koreans.
- Author:
Kyou Sup HAN
1
;
Sung Ha KANG
;
Yasuo FUKUMORI
;
Hirotoshi SHIBATA
;
Yasuto OKUBO
Author Information
1. Department of Clinical Pathology, Seoul National University College of Medicine, Seoul, College of Medicine, Korea.
- Publication Type:Original Article
- Keywords:
Molecular genetic polymorphism;
Cis-AB;
Korean;
PCR-RFLP;
PCR-SSCP
- MeSH:
Alleles;
Asian Continental Ancestry Group;
DNA;
Exons;
Genetics;
Humans;
Japan;
Korea;
Molecular Biology*;
Nucleotides;
Polymorphism, Genetic;
Transferases
- From:Korean Journal of Blood Transfusion
1997;8(1):85-91
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: Cis-AB is very rare among caucacians and found relatively often among Korean and Japanese populations. The molecular genetic characteristics of Japanese cis-AB were reported recently. All the Japanese cis-AB analyzed so far had identical polymorphisms. This work was performed to disclose the genetic polymorphisms in Korean cis-AB individuals. METHODS: DNA was extracted from 8 cis-AB Koreans. A and O transferase genes were amplified with GA0IN/GA14 allele-specific primer set, and B and cis-AB genes were amplified with GA0IN/GA13 allele-specific primer set. Restriction enzymes including BstPI, KpnI, BssHII, BsaHI, HapII, AluI, and MvaI were used for PCR-RFLP to find out the coexistence of O gene and to determine the nucleotides at nps 467, 526, 703, and 796. Exon 7 was divided into 2 regions and amplified to perform PCR-SSCP. RESULTS: All of the Korean cis-AB analyzed had identical genetic polymorphisms to those reported on Japanese cis-AB. PCR-RFLP with GA0IN/GA13 showed that all cis-AB samples had the allele with A-specific nucleotides at nps 526,703 and 796, and B-specific nucleotides at np 803. Other genetic polymorphisms showed that all of those with cis-AB allele had T at np 467 (leucine at amino acid position 156). CONCLUSION: Genetics polymorphisms reported on 29 Japanese cis-AB so far and 8 Koreans cis-AB analyzed in this study had identical mutations. This may imply that the cis-AB often found in Korea and Japan have single origin. It is plausible that a group of Korean people immigrated from the southwest part of Korea to the western part of Japan through a sea route in the past.
