The Genotype Frequencies of Platelet-Specific Antigens in Koreans.
- Author:
Dong Hee SEO
1
;
Sung Sup PARK
;
Dae Won KIM
;
Kyou Sup HAN
Author Information
1. Department of Clinical Pathology, Seoul National University College of Medicine, Seoul, Korea.
- Publication Type:Original Article
- Keywords:
Platelet-specific antigen;
Genotype;
Allele-specific polymerase chain reaction;
RFLP;
Gene frequency
- MeSH:
Antigens, Human Platelet*;
Asian Continental Ancestry Group;
Base Pairing;
Blood Platelets;
DNA;
Gene Frequency;
Genotype*;
Humans;
Isoantibodies;
Membrane Glycoproteins;
Polymerase Chain Reaction;
Polymorphism, Restriction Fragment Length;
Purpura;
Thrombocytopenia, Neonatal Alloimmune
- From:Korean Journal of Blood Transfusion
1997;8(1):93-102
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: The platelet-specific antigens which exist specifically on platelets have their antigenic determinant on platelet membrane glycoprotein. The alloantibodies against this antigens are responsible for neonatal alloimmune thrombocytopenia, posttransfusion purpura and platelet refractoriness in multitransfused patients. Discovering of the fact that the polymorphism of platelet-specific antigens is resulted from a single base pair substitution of genomic DNA stimulated studies on genotyping of platelet-specific antigens on various populations. This study was performed to investigate the genotype frequency of platelet-specific antigens in Koreans. METHODS: Using genomic DNA extracted from venous blood of 200 Koreans, genotype of seven platelet-specific antigen systems were determined through allele-specific polymerase chain reaction and restriction fragment length polymorphism. RESULTS: The genotype frequencies of HPA-1 were a+b- 98.0%, a+b+ 1.5%, a-b+ 0.5%; HPA-2, a+b- 85.5%, a+b+ 13.5%, a-b+ 1.0%; HPA-3, a+b- 28.5%, a+b+ 54.0%, a-b+ 17.5%; HPA-4, a+b- 98.0%, a+b+ 2.0%, a-b+ 0.0%; HPA-5, a+b- 95.5%, a+b+ 4.5%; HPA-6W, a+b- 96.0%, a+b+ 4.0%; HPA-7W were a+b- 100.0%, a+b+ 0.0%. CONCLUSION: The gene frequency of HPA-1b in Koreans was lower than that of Caucasian. As a whole, the genotype frequencies of platelet-specific antigens in Koreans were similar to those of the Japanese. However, we found one HPA-1(a-b+) among 200 Koreans, which is very rare in Japanese. This study will serve as a basic data for the study and management of the patients with diseases associated with platelet-specific antigens and antibody reactions in Koreans.
