Evaluation of COBE Spectra "Leukocyte Reduction System (LRSTM)" for the Production of Leukocyte-Reduced Platelets.
- Author:
Jong Seong CHOI
1
;
Dong Hee SEO
;
Hae Kyoung CHOUNG
;
Kum Hee LEE
;
Dae Won KIM
Author Information
1. Department of Clinical Pathology, Sungkyunkwan University College of Medicine, Samsung Medical Center, Seoul, Korea.
- Publication Type:Original Article
- Keywords:
COBE Spectra LRSTM;
platelet yield;
efficiency;
leukocytes contamination
- MeSH:
Bacterial Infections;
Blood Component Removal;
Blood Group Incompatibility;
Blood Platelets;
Humans;
Leukocytes;
Platelet Transfusion;
Plateletpheresis;
Tissue Donors
- From:Korean Journal of Blood Transfusion
1997;8(1):103-109
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: Leukocytes have been shown to be an undesirable contaminants in platelet transfusions because these contaminants may develop various adverse consequences. Current platelet products by plateletpheresis are heavily contaminated with leukocytes. Recently, new platelet apheresis system (COBE Spectra LRSTM) was designed to make it possible to collect platelets with very low leukocytes contamination. We evaluated the COBE Spectra LRSTM by comparing it with COBE Spectra. METHODS: Plateletpheresis procedures were performed on 75 normal donors; 45 procedures for COBE Spectra LRSTM and 30 procedures for COBE Spectra. We evaluated platelet yields, processing times, efficiency, and leukocytes content on two apheresis machines. RESULTS: Comparative results of COBE Spectra LRSTM with COBE Spectra were as follows: the mean processing time per unit was 97 min and 91 min, the efficiency per unit was 38.4 +/- 11.5% and 46.9 +/- 12.1%, the mean leukocytes contamination per unit was 6.1x104 and 2.1x106 respectively (p<0.01). The platelet yields per unit were 2.79 +/- 1.04x1011 with COBE Spectra LRSTM and 3.13 +/- 0.91x1011 with COBE Spectra (p>0.05). CONCLUSIONS: Platelet collections with COBE Spectra LRSTM demonstrated comparable platelet yields and strikingly low WBC contamination. This study indicate that the COBE Spectra LRSTM is an efficient and reliable system for the collection of platelets with very low residual WBC levels. It seems that leukocyte reduction filter for platelet products by COBE Spectra LRSTM is not necessary for further removal of leukocytes to prevent alloimmunization, non-hemolytic transfusion reactions, certain viral and bacterial infections.
