Protective Effect of Thiore doxin on Bovine Corneal Endothelial Cells Damaged by Oxidative Stresses.
- Author:
Dong Yeon LEE
1
;
Yun Song LEE
Author Information
1. Department of Ophthalmology, Pundang Jesaeng General Hospital, Sungnam, Korea.
- Publication Type:Original Article
- Keywords:
Reactive oxygen species;
Thioredoxin;
Corneal endothelial cell
- MeSH:
Antioxidants;
Corneal Diseases;
Corneal Edema;
Endothelial Cells*;
Endothelium, Corneal;
Escherichia coli;
Hydrogen Peroxide;
L-Lactate Dehydrogenase;
Oxidative Stress*;
Oxygen;
Reactive Oxygen Species;
Thioredoxins;
Ultraviolet Rays
- From:Journal of the Korean Ophthalmological Society
2000;41(10):2060-2068
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
Reactive oxygen species including hydrogen peroxide are known to induce morphological and functional derangement of corneal endothelium, thus causing loss of corneal deturgescence. Among the antioxidants, thioredoxin has been recently suggested to play an important role under oxidative stresses. The present study was undertaken to investigate the protective effect of thioredoxin, obtained from Escherichia coli, on cultured bovine endothelial cells against hydrogen peroxide and ultraviolet ray. Thioredoxin at concentrations of 1, 10 and 100 microgram/ml appeared to protect corneal endothelial cells from the reactive oxygen radical hydrogen peroxide as assessed by measuring the leakage of lactate dehydrogenase (LDH) and the reduction of 3-[4, 5-dimethyl-2-yl]-2, 5-diphenyl-tetrazolium bromide (MTT). In conclusion, thioredoxin may play an important physiological role (s) in protection of corneal endothelial cells which is continuously challenged by reactive oxygen species. Thus, It is natural that thioredoxin may have clinical implications in certain corneal disease such as corneal edema, which is known to be caused by reactive oxygen species.
