Culture of Nail Matrix Cells.
10.5021/ad.1997.9.4.242
- Author:
Jae Hak YOO
;
Hee Chul EUN
;
Yoon Ho LEE
- Publication Type:Original Article
- Keywords:
Hard keratins;
Nail matrix cells
- MeSH:
Biology;
Humans;
In Vitro Techniques;
Methods;
Skin
- From:Annals of Dermatology
1997;9(4):242-245
- CountryRepublic of Korea
- Language:English
-
Abstract:
BACKGROUND: In vitro epidermal keratinocytes(EKs) culture systems are widely used in studying skin biology and differentiation. Recently a monolayer culture method of nail matrix cells(NMCs) has been developed. OBJECTIVE: This study was designed to establish a method of culture of NMCs and compared its characteristics with those of EKs. METHODS: Human NMCs were isolated and cultured in a defined medium. Phase contrast microscopic examination, growth rate and ketatin expression were evaluated. RESULTS: In in vitro monolayer culture, NMCs had a similar growth pattern and morphological features as EKs. The synthesis of hard keratins was detected. CONCLUSION: These observations suggest that the NMCs culture model may be useful for the study of nail properties and the effects of drugs.
