Early Phase of UVB-induced GM-CSF Upregulation in Epithelial Cell Line is not Totally Dependent on IL-1α.
10.5021/ad.1997.9.4.246
- Author:
Kyoung Chan PARK
;
Ji Hwan WHANG
;
Sang Woong YOUN
;
Jong Seong AHN
;
Young Gull KIM
;
Woo Seok KOH
;
Kyu Han KIM
;
Jin Ho CHUNG
;
Jai Il YOUN
;
Hyun Chae JUNG
- Publication Type:Original Article
- Keywords:
Ultraviolet B;
IL-1α;
GM-CSF;
RT-PCR
- MeSH:
Antibodies;
Cell Line;
Epithelial Cells*;
Gene Expression;
Granulocyte-Macrophage Colony-Stimulating Factor*;
Humans;
Interleukin-1;
Keratinocytes;
RNA, Messenger;
Ultraviolet Rays;
Up-Regulation*
- From:Annals of Dermatology
1997;9(4):246-252
- CountryRepublic of Korea
- Language:English
-
Abstract:
BACKGROUND: It was demonstrated that ultraviolet(UV) B light induces the release of IL-1α in cultured human epithelial cell line and augmentation of GM-CSF production by UVB is reported to be mediated by IL-1α in the murine keratinocyte cell line Pam 212. OBJECTIVE: The purpose of this study was to evaluate the effects of UVB on kinetic profile of IL-1 and GM-CSF mRNA expression and to see whether synthesis of GM-CSF by UVB can be completely inhibited by blocking IL-1α mediated pathway. METHODS: We used a competitive RT-PCR for measuring cytokine gene expression in epithelial cell line after UV radiation. RESULTS: The IL-1α mRNA increased as early as 1h after UV irradiation, and then decreased at 3h after the irradiation. Thereafter, the response of IL-1α mRNA was upregulated with a second peak at 6h after the UV irradiation. However, mRNA for GM-CSF increased at 1h after UV light exposure and anti-IL-1α antibodies could only partially inhibit UV-augmented GM- CSF production. CONCLUSION: UVB induced GM-CSF production seemed to be mainly mediated by UVB induced IL-1α but these results suggest that UVB may also induce GM-CSF production through an IL-1α independent pathway.
