Biological Activities and Stability of a Standardized Pentacyclic Triterpene Enriched Centella asiatica Extract.
10.20307/nps.2016.22.1.20
- Author:
Panupong PUTTARAK
1
;
Adelheid BRANTNER
;
Pharkphoom PANICHAYUPAKARANANT
Author Information
1. Department of Pharmacognosy and Pharmaceutical Botany, Faculty of Pharmaceutical Sciences, Prince of Songkla University, Hat-Yai, Songkhla 90112, Thailand. pharkphoom.p@psu.ac.th
- Publication Type:Original Article
- Keywords:
Centella asiatica;
Pentacyclic triterpenes;
Antiinflammatory;
Antioxidant;
Tyrosinase inhibition;
Stability
- MeSH:
Alcoholics;
Centella*;
Humans;
Hydrogen-Ion Concentration;
Inhibitory Concentration 50;
Monophenol Monooxygenase;
Nitric Oxide;
Pentacyclic Triterpenes
- From:Natural Product Sciences
2016;22(1):20-24
- CountryRepublic of Korea
- Language:English
-
Abstract:
Pentacyclic triterpenes, mainly, asiatic acid, madecassic acid, asiaticoside, and madecassoside are the active constituents of Centella asiatica. A pentacyclic triterpene enriched C. asiatica extract (PRE) was prepared and standardized to contain a total pentacyclic triterpenes not less than 65% w/w. This work was focused on determination of antiinflammatory, antioxidant, and tyrosinase inhibitory activities of PRE and its stability. The PRE exhibited a satisfactory nitric oxide inhibitory effect, with an IC50 value of 64.6 µg/mL. In addition, the PRE inhibited tyrosinase enzyme activity with an IC50 value of 104.8 µg/mL. In contrast, the PRE possessed only weak antioxidant activity. The PRE was stable over a period of four months when stored as a dried powder but only in a well-closed container protected from light at 4 °C. An aqueous alcoholic solution of the PRE was stable at pH values of 5.8 and 7.0, but was not stable at a pH of 8.2. Preparations of the PRE in an aqueous solution should be performed in acidic or neutral conditions.
